PXD068953-1

PXD068953 is an original dataset announced via ProteomeXchange.

Title	Nearly complete redirection of insertion-type indel into recombination enhances knock-in and facilitates endogenous biomolecular condensate analysis
Description	Biomolecular condensate is crucial for cellular organization and function, yet its study faces significant technical challenges1,2. Cells often operate near a phase separation threshold, where even slight overexpression can profoundly alter condensate behavior3. Despite this sensitivity, many studies continue to rely on ectopic expression for in vivo measurements4. This reliance underscores the limitations of current knock-in (KI) techniques, where the inefficiency of homologous directed repair (HDR) is often outcompeted by the error-prone non-homologous end joining (NHEJ) pathway, frequently resulting in unwanted insertions and deletions (indels) rather than KI5. Newer base editing (BE) and prime editing (PE) technologies, though more precise, remain constrained5. BE only performs specific base substitutions, and PE is limited to short edits near PAM sequences. Neither can efficiently KI complex sequences needed for studying condensates. For example, modifying polyQ tract of ATXN2, a long repetitive sequence linked to neurodegeneration6,7, remains challenging. Besides, unintended off-target effects and chromosomal rearrangements have raised concerns about the safety of KI technology in practical applications. Therefore, a highly efficient and versatile KI technology is needed as a reliable tool for studying the intricate dynamics of biomolecular condensates.
HostingRepository	iProX
AnnounceDate	2025-09-27
AnnouncementXML	Submission_2025-09-29_02:29:16.460.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Boyi Chen
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-29 02:29:03	ID requested
⏵ 1	2025-09-29 02:29:16	announced

Dataset with its publication pending

submitter keyword: RecT7, biomolecular condensates, knock-in techniques

Wei Qin
contact affiliation	Tsinghua University
contact email	weiqin@tsinghua.edu.cn
lab head
Boyi Chen
contact affiliation	Tsinghua University
contact email	cby23@mails.tsinghua.edu.cn
dataset submitter

iProX dataset URI