PXD068912 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Arginine deprivation of ASS1-deficient cancers drives mistranslation and shared neoepitope production |
| Description | Arginine biosynthesis is frequently suppressed in human cancer due to loss of argininosuccinate synthetase 1 (ASS1) expression, rendering cancer cells reliant on extracellular arginine. This observation propelled the development of systemic arginine depletion strategies, which are clinically safe but showed limited efficacy. Here, we show that in conditions of arginine scarcity, cancer cells with low ASS1 expression resort to aberrant mRNA translation in the form of ribosomal frameshifts and amino acid misincorporations. While aberrant proteins originated from most arginine codons, the predominant effect was observed at AGA. This codon-preference is caused by a selective decrease of tRNAArg(UCU) levels after arginine deprivation, which is linked to METTL1-mediated tRNA-modification. Using proteomics and immunopeptidomics, we validated that arginine shortage induces aberrant protein and neoepitope production at the endogenous level. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-28 |
| AnnouncementXML | Submission_2026-04-28_10:19:07.458.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Onno Bleijerveld |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Astral; Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-28 16:41:16 | ID requested | |
| ⏵ 1 | 2026-04-28 10:19:08 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
Contact List
| Onno Bleijerveld |
|---|
| contact affiliation | Netherlands Cancer Institute Proteomics Facility |
| contact email | o.bleijerveld@nki.nl |
| lab head | |
| Onno Bleijerveld |
|---|
| contact affiliation | The Netherlands Cancer Institute |
| contact email | o.bleijerveld@nki.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068912
- Label: PRIDE project
- Name: Arginine deprivation of ASS1-deficient cancers drives mistranslation and shared neoepitope production
