PXD068848 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Human Calcitonin and Amylin receptor HDX-MS |
| Description | Receptor activity-modifying proteins (RAMPs) regulate the pharmacology, trafficking, and signaling of class B1 G protein-coupled receptors (GPCRs). The calcitonin receptor (CTR) interacts with each of the three RAMPs to form amylin receptors (AMYRs), which display distinct ligand preferences and signaling outputs. While cryo-electron microscopy has described AMYR structures in active conformations, less is known about how RAMPs influence CTR dynamics in the absence of ligand. In this study, we applied hydrogen–deuterium exchange mass spectrometry (HDX-MS) to examine conformational dynamics of the CTR alone and in complex with RAMP1, RAMP2, or RAMP3 in their apo states. The data show that RAMPs alter the flexibility of several CTR regions, including extracellular loops, transmembrane helices, and intracellular segments associated with G protein binding. In addition, each RAMP subtype displays characteristic dynamic features within its transmembrane and C-terminal domains. These HDX-MS datasets provide mechanistic insight into how RAMPs modulate CTR structure and offer a resource that links static structural models with functional pharmacology. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-08 |
| AnnouncementXML | Submission_2026-06-07_18:26:57.337.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tracy Josephs |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Synapt MS |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-25 17:15:39 | ID requested | |
| ⏵ 1 | 2026-06-07 18:26:58 | announced | |
Publication List
| Fairweather CJ, Zhang X, Fernando CD, Garama DJ, Sexton PM, Wootten D, Josephs TM, Conformational Dynamics of Amylin Receptors Revealed by Hydrogen-Deuterium Exchange Mass Spectrometry. J Am Chem Soc, 148(16):16702-16712(2026) [pubmed] |
| 10.1021/jacs.5c20644; |
Keyword List
| ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS) |
| submitter keyword: calcitonin recepto,HDX-MS, recepror activity modifying protein (RAMP), GPCR, amylin receptor |
Contact List
| Tracy Maree Josephs |
|---|
| contact affiliation | Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Parkville, Victoria, Australia |
| contact email | tracy.josephs@monash.edu |
| lab head | |
| Tracy Josephs |
|---|
| contact affiliation | Monash University |
| contact email | tracy.josephs@monash.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD068848 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068848
- Label: PRIDE project
- Name: Human Calcitonin and Amylin receptor HDX-MS
