PXD068844-1

PXD068844 is an original dataset announced via ProteomeXchange.

Title	Coimmunoprecipitation and following mass spectrometry analysis of EhGEFM
Description	To find out the binding partners of EhGEFM, crosslinking and coimmunoprecipitation were performed. Then, eluted samples were submitted to Mass Spectrometry and Proteomics Core Facility, Johns Hopkins University School of Medicine and analyzed by mass spctrometry (shotgun proteomics). The coimmunoprecipitation and following analysis were conducted twice. You can find data of the HA-EhGEFM in the first trial from "2024_8_55_44_PM_FileSize_988042288_Byte_F015729.mzid_2024_8_55_44_PM_File_Size__988042288__Byte__F015729.MGF", "2024_8_55_44_PM_FileSize_988042288_Byte_F015729.mzid", and "TB-LI_CS-240315_TsukuiK_KT_1_10p.RAW". You can find data of pEhEx-HA (mock) in the first trial from "2024_12_11_27_AM_FileSize_837709430_Byte_F015730.mzid_2024_12_11_27_AM_File_Size__837709430__Byte__F015730.MGF", "2024_12_11_27_AM_FileSize_837709430_Byte_F015730.mzid", and "TB-LI_CS-240315_TsukuiK_KT_2_10p.RAW". You can find the data of the HA-EhGEFM in the second trial from 2024_2_38_07_PM_FileSize_1529844717_Byte_F016345.mzid_2024_2_38_07_PM_File_Size__1529844717__Byte__F016345.MGF", "2024_2_38_07_PM_FileSize_1529844717_Byte_F016345.mzid", "RO-LUM_CS-240430_TsukuiK_KT_HA-RhoGEF-2.msf", "RO-LUM_CS-240430_TsukuiK_KT_HA-RhoGEF-2.mzML", and "RO-LUM_CS-240430_TsukuiK_KT_HA-RhoGEF.RAW". You can find data of pEhEx-HA (mock) in the second trial from "2024_5_13_15_PM_FileSize_1498194810_Byte_F016346.mzid_2024_5_13_15_PM_File_Size__1498194810__Byte__F016346.MGF", "2024_5_13_15_PM_FileSize_1498194810_Byte_F016346.mzid", "RO-LUM_CS-240430_TsukuiK_KT_pEhExHA-2.msf", "RO-LUM_CS-240430_TsukuiK_KT_pEhExHA-2.mzML", and "RO-LUM_CS-240430_TsukuiK_KT_pEhExHA.RAW"
HostingRepository	PRIDE
AnnounceDate	2026-05-16
AnnouncementXML	Submission_2026-05-16_08:16:25.238.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD068844
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Misato Shimoyama
SpeciesList	scientific name: Entamoeba histolytica HM-1:IMSS; NCBI TaxID: NEWT:294381;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-25 16:48:51	ID requested
⏵ 1	2026-05-16 08:16:26	announced

10.6019/PXD068844;

10.1242/jcs.264490;

Shimoyama M, Nakada-Tsukui K, Nozaki T, A RhoGEF activates EhRacM to selectively promote motility without affecting macropinocytosis in Entamoeba histolytica. J Cell Sci, ():(2026) [pubmed]

submitter keyword: Rho, EhGEFM,Entamoeba histolytica, co-IP, EhRacM, RhoGEF

Tomoyoshi Nozaki
contact affiliation	Department of Biomedical Chemistry, The University of Tokyo
contact email	nozaki@m.u-tokyo.ac.jp
lab head
Misato Shimoyama
contact affiliation	The University of Tokyo
contact email	shimoyama-misato142@g.ecc.u-tokyo.ac.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD068844

PRIDE project URI

• PRIDE
  1. PXD068844
     1. Label: PRIDE project
     2. Name: Coimmunoprecipitation and following mass spectrometry analysis of EhGEFM