PXD068820 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Co-Immunoprecipitation of Ca. Lokiarchaeum ossiferum Actin |
| Description | This dataset was generated using a crosslinking immunoprecipitation–mass spectrometry (IP–MS) approach to identify proteins interacting with Actin. Cultured cells were mildly fixed with formaldehyde to preserve protein–protein interactions, quenched, and lysed under gentle detergent conditions. Actin-associated complexes were immunoprecipitated using antibody-coupled magnetic beads, while IgG beads served as negative controls. The recovered proteins were then subjected to proteomic analysis to define the Actin interactome and distinguish specific interaction partners from background binders. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-13 |
| AnnouncementXML | Submission_2026-08-13_05:08:21.001.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Markus Hartl |
| SpeciesList | scientific name: Candidatus Lokiarchaeum sp. B-35; NCBI TaxID: NEWT:2951803; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-25 01:31:09 | ID requested | |
| ⏵ 1 | 2026-08-13 05:08:21 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Asgard, eukaryogenesis, actin, archaea, immunoprecipitation |
Contact List
| Christa Schleper |
|---|
| contact affiliation | Department of Functional and Evolutionary Ecology; University of Vienna |
| contact email | christa.schleper@univie.ac.at |
| lab head | |
| Markus Hartl |
|---|
| contact affiliation | Max Perutz Labs, Mass Spectrometry Facility, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 9, Vienna, Austria |
| contact email | proteomics@maxperutzlabs.ac.at |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD068820 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068820
- Label: PRIDE project
- Name: Co-Immunoprecipitation of Ca. Lokiarchaeum ossiferum Actin
