PXD068810-1

PXD068810 is an original dataset announced via ProteomeXchange.

Title	Plasma Exosomal Proteomics: Unveiling Novel Biomarkers for Ischemic Stroke in the Context of Diabetes
Description	Diabetes increases the risk of ischemic stroke and mortality. Exosomes, which are vesicles abundant of not only nucleic acids but also proteins, transmit stable cellular information and traverse the blood-brain barrier, emerge as promising biomarkers for brain injury and non-invasive monitoring. Investigating exosome-proteomics might unveil innovative therapeutic strategies for improving outcomes in diabetic stroke patients. Exosomes were isolated from the plasma of acute ischemic stroke patients with and without type 2 diabetes (n=10 each). Electron microscopy and flow cytometry were employed to analyze exosome morphology and markers, while nanoparticle tracking assessed concentration and size. Label-free quantitative proteomics and bioinformatics identified novel prognostic biomarkers. The CIBERSORTx and BRETIGEA tools were respectively used to elucidate immune and brain cell-specific protein expression patterns from exosomes. Diabetic stroke patients exhibited elevated fasting glucose, HbA1c, and neutrophil levels. Proteomic profiling revealed 95 differentially expressed proteins (21 up-regulated, 74 down-regulated). Functional enrichment analyses (GO and KEGG) associated diabetic stroke with disrupted oxygen/nitric oxide transport, cellular and glucose metabolism, and reactive oxygen species response. Protein-protein interaction network analysis highlighted the recombinant hemoglobin family (HBA1, HBA2, HBB), human β defensin (HBD), phosphofructokinase-1 liver type (PFKL), and aldehyde dehydrogenase 3 family member A2 (ALDH3A2) as key regulators. Diabetes altered exosomal contributions, diminishing signals from CD4 memory T cells and brain endothelial cells, while enhancing those from follicular helper T cells. These findings underscore the potential of exosome-based diagnostics and therapeutics for diabetic stroke, paving the way for innovative therapeutic strategies.
HostingRepository	PRIDE
AnnounceDate	2026-04-13
AnnouncementXML	Submission_2026-04-12_17:03:20.227.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haiping Zhao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	carboxylated residue
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-24 21:19:41	ID requested
⏵ 1	2026-04-12 17:03:21	announced

10.1016/j.brainresbull.2026.111884;

Jiang Q, Peng G, Kong Q, Zhou Y, Zhao H, Geng X, Plasma exosomal proteomics: Unveiling novel biomarkers for ischemic stroke in the context of diabetes. Brain Res Bull, 239():111884(2026) [pubmed]

submitter keyword: Diabetes;Exosomes;Proteomics

Haiping Zhao
contact affiliation	Institute of Cerebrovascular Diseases Research, Xuanwu Hospital of Capital Medical University, Beijing, China
contact email	zhaohaiping1980@163.com
lab head
Haiping Zhao
contact affiliation	Institute of Cerebrovascular Diseases Research, Xuanwu Hospital of Capital Medical University
contact email	zhaohaiping1980@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068810
     1. Label: PRIDE project
     2. Name: Plasma Exosomal Proteomics: Unveiling Novel Biomarkers for Ischemic Stroke in the Context of Diabetes