PXD068796 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | SARS-CoV-2 Nsp3 interactome in Caco-2 cells during authentic infection |
| Description | The nonstructural protein 3 (Nsp3) of SARS-CoV-2 is a large, multi-domain protein that localizes to double-membrane vesicles (DMVs), the physical site where SARS-CoV-2 (and other coronaviruses) replicate inside mammalian cells. One of the domains of Nsp3 is PLpro (papain-like protease), which harbors enzymatic activities (i.e., protease, deubiquitinase, and deISGylase). To identify human cellular proteins that bind Nsp3, we performed LC-MS/MS analysis of affinity-purified Nsp3 from SARS‑CoV‑2–infected Caco-2 (human intestinal epithelial) cells. This dataset identified numerous Nsp3-interacting proteins that play key roles in various host cellular processes, including metabolism and innate immunity. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-06 |
| AnnouncementXML | Submission_2026-05-05_16:46:23.697.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Junji Zhu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro 2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-24 17:17:15 | ID requested | |
| ⏵ 1 | 2026-05-05 16:46:24 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Nsp3,SARS-CoV-2, coronavirus, interactome, Caco-2, LC-MS/MS |
Contact List
| Michaela Gack |
|---|
| contact affiliation | Florida Research and Innovation Center, Cleveland Clinic, Port Saint Lucie, FL 34987, USA |
| contact email | GACKM@ccf.org |
| lab head | |
| Junji Zhu |
|---|
| contact affiliation | Florida Research and innovation center, Cleveland Clinic |
| contact email | zhuj6@ccf.org |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD068796 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068796
- Label: PRIDE project
- Name: SARS-CoV-2 Nsp3 interactome in Caco-2 cells during authentic infection
