PXD068767 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | MASP-II for Mapping the Whole-tissue Spatial Proteome and the Intra-brain Distribution of Monoclonal Antibodies |
| Description | Spatial proteomics at whole-tissue level provides critical insights into region-specific biological regulations but remains challenging. Previously, we introduced the Micro-scaffold Assisted Spatial Proteomics (MASP) technique, permitting whole-tissue mapping. While promising, this prototype required substantial improvements in spatial resolution, practicality, and throughput for practical application. Here we present a next-generation MASP technique (MASP-II) featuring i) a new design of hexagonal-micro-wells fabricated with optimized Projection Micro-Stereolithography (PµSL) 3D-printing, achieving high spatial resolution, sampling robustness and mechanical strength for reproducibly compartmentalizing even tough tissues; ii) enhanced throughput/effectiveness in sample preparation and LC-MS analysis with high quantitative quality. Using MASP-II, we generated in-depth, whole-tissue maps for >6,000 proteins in mouse brains, with thoroughly validated mapping accuracy. The improved resolution of MASP-II revealed critical details that were previously absent in maps by its prototype, enabling precise depiction of protein distribution heterogeneity. This technique enabled the discovery of many novel regionally enriched proteins across brain structures. Applying MASP-II to study the intra-brain distribution of intracerebroventricularly-dosed antibody drugs and related proteins revealed novel insights into antibody distribution mechanisms and local effects induced by drug treatment. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-24 |
| AnnouncementXML | Submission_2026-08-24_09:12:41.148.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Min Ma |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-24 06:54:06 | ID requested | |
| ⏵ 1 | 2026-08-24 09:12:41 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: MASP, whole tissue mapping, accurate protein distribution, in-depth spatial proteomics, mAb intra-brain distribution |
Contact List
| Jun Qu |
|---|
| contact affiliation | Department of Pharmaceutical Sciences, University at Buffalo, US |
| contact email | junqu@buffalo.edu |
| lab head | |
| Min Ma |
|---|
| contact affiliation | University at Buffalo |
| contact email | minma@buffalo.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD068767 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068767
- Label: PRIDE project
- Name: MASP-II for Mapping the Whole-tissue Spatial Proteome and the Intra-brain Distribution of Monoclonal Antibodies
