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PXD068760-1

PXD068760 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Tandem affinity purification of SHFL-interacting proteins in 293 cells
Description	HA-Flag-SHFL was expressed in HEK293 cells in a doxycycline-inducible manner. The cell lysates were first treated with the nuclease benzonase to disrupt RNA- or DNA-mediated protein-protein interactions. The proteins were sequentially purified with anti-HA and anti-Flag conjugated beads. Purified proteins was subjected to Mass spectrometry analysis combined with label-free quantification.
HostingRepository	iProX
AnnounceDate	2025-09-24
AnnouncementXML	Submission_2025-09-24_02:40:47.259.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yongle Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-24 02:39:56	ID requested
⏵ 1	2025-09-24 02:40:47	announced

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: TAP, SHFL, 293A

Contact List

Guangxia Gao
contact affiliation	nstitute of Biophysics, Chinese Academy of Sciences
contact email	gaogx@moon.ibp.ac.cn
lab head
Yongle Wang
contact affiliation	nstitute of Biophysics, Chinese Academy of Sciences
contact email	talentony@gmail.com
dataset submitter

Full Dataset Link List

iProX dataset URI