PXD068732 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | E. coli slow recovery from growth arrest |
| Description | Bacteria shut down protein synthesis under stress, but how they resume growth remains unclear. We used an E. coli strain lacking a ribosome maturation factor RimM, which exhibits prolonged growth arrest, as a model to study bacterial recovery. Loss of this factor delays 30S ribosomal subunit assembly more than other maturation factors such as RbfA. Mass spectrometry and cryo-EM analyses revealed disrupted assembly of the 30S head domain, increased binding of initiation factors (IF1 and IF3), and recruitment of a silencing factor RsfS to the 50S subunit, collectively preventing premature 70S formation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-04 |
| AnnouncementXML | Submission_2026-03-04_14:18:34.240.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Pavel Roudnický |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: NEWT:562; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-23 03:21:38 | ID requested | |
| ⏵ 1 | 2026-03-04 14:18:34 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Ribosome assembly, LC-MSMS, proteomics, timfTOF HT, Bacteria, anti-association, E.coli |
Contact List
| Zbynek Zdrahal |
|---|
| contact affiliation | Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic |
| contact email | zdrahal@sci.muni.cz |
| lab head | |
| Pavel Roudnický |
|---|
| contact affiliation | Masaryk University |
| contact email | p.roudnicky@mail.muni.cz |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068732
- Label: PRIDE project
- Name: E. coli slow recovery from growth arrest
