PXD068726 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Multilayered regulation of TORC1 signaling by Ait1, Gcn2, and SEAC/GATOR during nitrogen limitation and starvation |
| Description | The Target of Rapamycin kinase Complex I (TORC1) is a central hub in the cell growth and metabolic control network of eukaryotes. How its upstream regulators cooperate to tune signaling across environmental conditions remains unclear. Here, we combine phosphoproteomics, TORC1 activity assays, and targeted genetic perturbations to dissect TORC1 regulation in Saccharomyces cerevisiae during transitions from a high-quality nitrogen source (glutamine) to a low-quality nitrogen source (proline), and on to complete nitrogen starvation. In proline medium, Ait1 and Gcn2 attenuate TORC1 activity, establishing a partially inhibited “Low Nitrogen Adaptive” state marked by extensive metabolic reprogramming without growth arrest. In contrast, during nitrogen starvation, SEAC, Ait1, and Gcn2 cooperate to drive TORC1 into a fully inhibited state, triggering widespread dephosphorylation of its downstream targets and entry into quiescence. Our results define a multilayered regulatory circuit that governs graded TORC1 control—a design likely conserved across eukaryotes. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-14 |
| AnnouncementXML | Submission_2025-11-13_20:12:51.149.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD068726 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Paul Langlais |
| SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932; |
| ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-23 01:01:58 | ID requested | |
| ⏵ 1 | 2025-11-13 20:12:51 | announced | |
Publication List
Keyword List
| submitter keyword: TORC1, Quiescence, SEAC/GATOR, Nitrogen starvation, Gcn2, Ait1, Nutrient sensing, mTOR |
Contact List
| Andrew Capaldi |
|---|
| contact affiliation | Professor, Molecular and Cellular Biology University of Arizona |
| contact email | capaldi@arizona.edu |
| lab head | |
| Paul Langlais |
|---|
| contact affiliation | University of Arizona |
| contact email | langlais@deptofmed.arizona.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD068726 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068726
- Label: PRIDE project
- Name: Multilayered regulation of TORC1 signaling by Ait1, Gcn2, and SEAC/GATOR during nitrogen limitation and starvation
