PXD068722-1

PXD068722 is an original dataset announced via ProteomeXchange.

Title	The antibacterial effect and molecular mechanism of magnolol on Aeromonas hydrophila
Description	Freshwater aquaculture is threatened by Aeromonas hydrophila, whose multidrug resistance limits the efficacy of antibiotics. Here, we investigated the antibacterial mechanisms of magnolol, a bioactive compound from Houpoea officinalis. Magnolol strongly inhibited A. hydrophila growth, impaired motility by affecting flagellar function, and disrupted membrane integrity. Proteomic analysis at a sublethal concentration revealed extensive remodeling of bacterial protein expression, with the sodium/glycine symporter GlyP markedly upregulated, suggesting disruption of glycine homeostasis. GO enrichment indicated enrichment of membrane-associated proteins and activation of envelope stress responses. Gene-deletion mutants further identified NagR, a GntR-family regulator of N-acetylglucosamine metabolism, as a potential target. ∆nagR grew better under magnolol stress, while molecular docking confirmed stable hydrogen bond interactions with NagR. Together, magnolol inhibits growth, motility, and membrane stability, while targeting NagR and disturbing metabolic homeostasis. These findings elucidate magnolol’s multifaceted antibacterial mechanisms and highlight its potential as a natural antimicrobial for aquaculture pathogen control and food safety improvement.
HostingRepository	iProX
AnnounceDate	2025-09-23
AnnouncementXML	Submission_2025-09-22_23:43:28.570.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xin Chen
SpeciesList	scientific name: Aeromonas hydrophila subsp. hydrophila ATCC 7966; NCBI TaxID: 380703;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-22 23:43:15	ID requested
⏵ 1	2025-09-22 23:43:28	announced

Dataset with its publication pending

submitter keyword: magnolol, A. hydrophila, proteomics, bactericidal mechanism, NagR

Xiangmin Lin
contact affiliation	Fujian Agriculture and Forestry University
contact email	xiangmin@fafu.edu.cn
lab head
Xin Chen
contact affiliation	Fujian Agriculture and Forestry University
contact email	2698584788@qq.com
dataset submitter

iProX dataset URI