PXD068715-1

PXD068715 is an original dataset announced via ProteomeXchange.

Title	Exploring the phosphor-proteome in B16 cells wild type cells or TAK1 CRISPR KO cells upon exposure to combined TNF and Interferon Gamma stimulation.
Description	Global phosphoproteomic profiling of wild-type and TAK1-deficient B16F10 cells treated with TNF (10ng/ml) and IFNγ (1ng/ml) for one hour. The analysis reveals that TAK1 loss selectively disrupts phosphorylation of NF-κB and MAPK pathway components, including RELA, RIPK2, and MAP3K8, while STAT1-associated phosphorylation events remain intact, indicating that IFNγ–STAT1 signaling is TAK1 independent. Stratification of phosphosites highlights TAK1-sensitive sites, which are induced in wild-type but lost in TAK1 knockout cells, and TAK1-independent sites, which remain unaffected by TAK1 loss. Notably, phosphorylation of RIPK1 was altered: the inhibitory S25 site was reduced in TAK1-deficient cells, while a cluster of sites spanning Y309–S415 was more prominently phosphorylated, consistent with enhanced pro-death signaling. Together, these data show that TAK1 sustains NF-κB–driven survival signaling while restraining RIPK1 activation, thereby acting as a molecular switch that biases TNF and IFNγ signaling toward cytoprotection; its loss rewires cytokine signaling toward apoptosis.
HostingRepository	PRIDE
AnnounceDate	2025-11-10
AnnouncementXML	Submission_2025-11-09_21:43:20.741.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chloe Gerak
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-22 19:45:53	ID requested
⏵ 1	2025-11-09 21:43:21	announced

Dataset with its publication pending

submitter keyword: TNF,B16 cells, Interferon Gamma, mouse

Stephin Vervoort
contact affiliation	Lab head, WEHI, Melbourne, Australia
contact email	vervoort.s@wehi.edu.au
lab head
Chloe Gerak
contact affiliation	Walter and Eliza Hall Institute
contact email	gerak.c@wehi.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068715
     1. Label: PRIDE project
     2. Name: Exploring the phosphor-proteome in B16 cells wild type cells or TAK1 CRISPR KO cells upon exposure to combined TNF and Interferon Gamma stimulation.