PXD068702-1

PXD068702 is an original dataset announced via ProteomeXchange.

Title	Proteome Analyses of Human NK Cells after alloSCT Challenged byAspergillus fumigatus
Description	We collected longitudinally human NK cells from patients after alloSCT (at days +90, +120, +180), to identify any potential functional NK cell defects on a protein level. We have focused our analysis on whole proteome patterns by analyzing patient NK cells stimulated with A. fumigatus or left unstimulated, in comparison to those obtained from healthy controls. In parallel, a broad collection of clinical data from these patients was archived. This data revealed specific, quite different protein patterns, depending on the status of the NK cells. We were able to define significant differences in the proteome composition of NK cell isolated from alloSCT recipients compared to healthy controls. The majority of these differences were present until day +180. Fungal stimulation of NK cells led initially to markedly differential proteomes while at day +180 only a small number of proteins were differentially expressed between A. fumigatus stimulated NK cells from alloSCT recipients compared to healthy controls. Furthermore, we were able to confirm our proteome data by flow cytometry.
HostingRepository	PRIDE
AnnounceDate	2026-05-01
AnnouncementXML	Submission_2026-05-01_08:08:28.242.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD068702
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thomas Krüger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Neosartorya fumigata (Aspergillus fumigatus); NCBI TaxID: NEWT:746128;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-22 16:08:12	ID requested
⏵ 1	2026-05-01 08:08:28	announced

10.6019/PXD068702;

submitter keyword: Aspergillus fumigatus, NK cells,alloSCT, LC-MS/MS

Axel A. Brakhage
contact affiliation	Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology / Hans Knöll Institute Jena (Leibniz-HKI), Jena, Germany
contact email	axel.brakhage@leibniz-hki.de
lab head
Thomas Krüger
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact email	thomas.krueger@leibniz-hki.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD068702

PRIDE project URI

• PRIDE
  1. PXD068702
     1. Label: PRIDE project
     2. Name: Proteome Analyses of Human NK Cells after alloSCT Challenged byAspergillus fumigatus