PXD068682-1

PXD068682 is an original dataset announced via ProteomeXchange.

Title	In human T-cell acute lymphoblastic leukemia (T-ALL), β-hydroxybutyrate dehydrogenase 1, the metabolic enzyme responsible for cell-autonomous BHB synthesis shows elevated protein expression without a corresponding increase at the mRNA level. We reasoned that BDH1 is deregulated is dysregulated post-transcriptionally and sought to identify the regulatory factors that sustain its aberrant protein expression.
Description	In human T-cell acute lymphoblastic leukemia (T-ALL), β-hydroxybutyrate dehydrogenase 1, the metabolic enzyme responsible for cell-autonomous BHB synthesis shows elevated protein expression without a corresponding increase at the mRNA level. We reasoned that BDH1 is deregulated is dysregulated post-transcriptionally and sought to identify the regulatory factors that sustain its aberrant protein expression.To identify BDH1 interacting proteins, total cellular proteins extracted from T-ALL cells overexpressing either the pHAGE vector or pHAGE-BDH1 were subjected to immunoprecipitation and were subjected to LC-MS.
HostingRepository	PRIDE
AnnounceDate	2025-12-09
AnnouncementXML	Submission_2025-12-09_03:35:13.814.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jin Xu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-22 06:01:56	ID requested
⏵ 1	2025-12-09 03:35:14	announced

Dataset with its publication pending

submitter keyword: T-ALL-Human-LC-MS

Hudan Liu
contact affiliation	Medical Research Institute Wuhan University
contact email	hudanliu@whu.edu.cn
lab head
Jin Xu
contact affiliation	Wuhan university
contact email	xujin25@whu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068682
     1. Label: PRIDE project
     2. Name: In human T-cell acute lymphoblastic leukemia (T-ALL), β-hydroxybutyrate dehydrogenase 1, the metabolic enzyme responsible for cell-autonomous BHB synthesis shows elevated protein expression without a corresponding increase at the mRNA level. We reasoned that BDH1 is deregulated is dysregulated post-transcriptionally and sought to identify the regulatory factors that sustain its aberrant protein expression.