PXD068666-1
PXD068666 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of membrane-bound proteins interacting with PLK2 |
| Description | 293T cells were transferred with PLK2. Membrane-associated proteins were collected and isolated. The supernatants containing solubilized membrane and membrane-associated proteins were collected and incubated. Supernatants from both extraction steps were pooled and incubated with Dynabeads Protein A/G preconjugated with the appropriate antibody. IP products were reduced with 20 mM dithiothreitol, followed by alkylation. Proteins were digested with trypsin. Peptides were desalted using STAGE TIP before downstream mass spectrometry analysis. MS experiments were conducted using a nanoscale ultra-high-performance liquid chromatography system connected to an Orbitrap Q-Exactive mass spectrometer equipped with a nanoelectrospray source. Raw data were processed using Proteome Discoverer, and MS/MS spectra were searched against the reviewed Swiss-Prot human proteome database. |
| HostingRepository | iProX |
| AnnounceDate | 2025-09-22 |
| AnnouncementXML | Submission_2025-09-22_02:34:28.873.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chenbo Yang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | phosphorylated residue |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-09-22 02:33:41 | ID requested | |
| ⏵ 1 | 2025-09-22 02:34:29 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: 293T, IP-MS, membrane protein, PLK2 substrate |
Contact List
| Jing Xue | |
|---|---|
| contact affiliation | Institute of Laboratory Animal Science (Beijing), Chinese Academy of Medical Sciences and Peking Union Medical College |
| contact email | xuejing@cnilas.com |
| lab head | |
| Chenbo Yang | |
| contact affiliation | Institute of Laboratory Animal Science (Beijing), Chinese Academy of Medical Sciences and Peking Union Medical College |
| contact email | yc2844165492@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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