PXD068664 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Targeting PURPL RNA Enabled Rejuvenation of Senescence Cells via Epigenetic Reprogramming |
| Description | Cellular senescence is a fundamental driver of ageing and age-related diseases, characterized by irreversible growth arrest and profound epigenetic alterations. While long non-coding RNAs (lncRNAs) have emerged as key regulators of senescence, their potential for senescent cell rejuvenation remains unexplored. Here, we identify the ageing-associated lncRNA PURPL as an epigenetic regulator that controls cellular rejuvenation through H3K9me3-mediated transcriptional silencing. CRISPRi-mediated PURPL depletion produces striking rejuvenation effects, resulting in restored youthful cell morphology, as well as suppression of senescence markers such as p21 and SA-β-gal. Conversely, PURPL overexpression accelerates cellular senescence, recapitulating the transcriptional and phenotypic hallmarks of ageing. Mechanistically, nuclear-localized PURPL regulates H3K9me3 deposition at 411 genomic loci including SERPINE1 (PAI-1) and EGR1, which are key senescence drivers. PURPL-mediated H3K9me3 loss at these loci derepresses their transcription, establishing a pro-senescence gene expression program. These findings reveal that PURPL is an epigenetic modulator of senescence and highlight its potential as a therapeutic target for age-related pathologies. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-10-27 |
| AnnouncementXML | Submission_2025-10-26_17:13:33.121.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | wenkai yi |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-22 02:30:48 | ID requested | |
| ⏵ 1 | 2025-10-26 17:13:34 | announced | |
Publication List
| 10.1186/s12967-025-07208-5; |
| Wang J, Yang X, Su X, Yi W, Sun W, Wei J, Ning YQ, Yan J, Targeting PURPL RNA enabled rejuvenation of senescence cells via epigenetic reprogramming. J Transl Med, 23(1):1127(2025) [pubmed] |
Keyword List
| submitter keyword: PURPL |
| RNA binding protein |
| CARPID |
Contact List
| Jian Yan |
|---|
| contact affiliation | City University of Hong Kong |
| contact email | jian.yan@cityu.edu.hk |
| lab head | |
| wenkai yi |
|---|
| contact affiliation | City University of Hong Kong |
| contact email | kaiyi2-c@my.cityu.edu.hk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068664
- Label: PRIDE project
- Name: Targeting PURPL RNA Enabled Rejuvenation of Senescence Cells via Epigenetic Reprogramming
