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PXD068638-1
PXD068638 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Lipocalin-2 Attenuates Vascular Calcification by Stabilizing Acadvl to Enhance Fatty Acid Oxidation |
| Description | Vascular calcification (VC) is a critical contributor to cardiovascular mortality, driven by osteogenic differentiation of vascular smooth muscle cells (VSMCs) under hyperphosphatemia, yet molecular mechanisms remain incompletely defined. This study investigates the role of lipocalin-2 (LCN2) in regulating VC through both clinical and experimental approaches. We found LCN2 expression was significantly downregulated in calcified radial arteries from chronic kidney disease (CKD) patients and in VSMCs under calcifying conditions. Functionally, recombinant LCN2 protein or LCN2 overexpression attenuated calcification in VSMCs, arterial rings, and a CKD-associated VC mouse model, while LCN2 knockdown exacerbated these effects. Multi-omics analysis revealed that LCN2 enhanced fatty acid β-oxidation (FAO), and pharmacological inhibition of FAO accelerated calcification. Mechanistic studies using co-immunoprecipitation mass spectrometry combined with computational modeling identified a direct and stable interaction between LCN2 and very long-chain acyl-CoA dehydrogenase (Acadvl), a key mitochondrial FAO enzyme. We demonstrated that LCN2 stabilized Acadvl and enhanced its expression, while Acadvl knockdown abolished LCN2's protective effects against VC. These findings establish the LCN2-Acadvl axis as a novel regulatory mechanism mitigating VC through enhancing FAO, revealing promising therapeutic targets for vascular calcification pathology. |
| HostingRepository | iProX |
| AnnounceDate | 2025-09-19 |
| AnnouncementXML | Submission_2026-07-01_00:57:54.870.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xiulin Li |
| SpeciesList | scientific name: Rattus norvegicus; NCBI TaxID: 10116; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-09-22 00:04:38 | ID requested | |
| ⏵ 1 | 2026-07-01 00:57:55 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: LCN2, Vascular Calcification, fatty acid β oxidation, Acadvl, vascular smooth muscle cells |
Contact List
| Mi Liu | |
|---|---|
| contact affiliation | The Eighth Affiliated Hospital, Southern Medical University (The First People’ s Hospital of Shunde, Foshan) |
| contact email | liumi@smu.edu.cn |
| lab head | |
| Xiulin Li | |
| contact affiliation | The Eighth Affiliated Hospital, Southern Medical University (The First People’ s Hospital of Shunde, Foshan) |
| contact email | xiulinl99@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
