PXD068578-1

PXD068578 is an original dataset announced via ProteomeXchange.

Title	An RNA Splicing System that Excises DNA Transposons from Animal mRNAs
Description	All genomes harbor mobile genetic parasites called transposable elements (TEs). Here we describe a system, which we term SOS splicing, that protects C. elegans and human genes from DNA transposon-mediated disruption by excising these TEs from host mRNAs. SOS splicing, which operates independently of the spliceosome, is a pattern recognition system triggered by base- pairing of inverted terminal repeat elements, which are a defining feature of the DNA transposons. We identify three factors required for SOS splicing in both C. elegans and human cells; AKAP17A, which binds TE-containing mRNAs; the RNA ligase RTCB; and CAAP1, which bridges RTCB and AKAP17A, allowing RTCB to ligate mRNA fragments generated by TE excision. We propose that SOS splicing is a novel, conserved, and RNA structure-directed mode of mRNA splicing and that one function of SOS splicing is to genetically buffer animals from the deleterious effects of TE- mediated gene perturbation.
HostingRepository	PRIDE
AnnounceDate	2025-09-22
AnnouncementXML	Submission_2025-09-22_08:24:36.018.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Nardone
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-19 07:59:14	ID requested
⏵ 1	2025-09-22 08:24:36	announced

Dataset with its publication pending

submitter keyword: Affinity Purification

Christopher Nardone
contact affiliation	Harvard Medical School, Department of Cell Biology Howard Hughes Medical Institute
contact email	christopher_nardone@hms.harvard.edu
lab head
Christopher Nardone
contact affiliation	Harvard Medical School
contact email	christopher_nardone@hms.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068578
     1. Label: PRIDE project
     2. Name: An RNA Splicing System that Excises DNA Transposons from Animal mRNAs