PXD068573 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic validation of saprophyticin S (SapS), a novel leaderless bacteriocin from Staphylococcus saprophyticus P1CEA4 |
| Description | This project reports the identification and characterization of saprophyticin S (SapS), a novel leaderless bacteriocin produced by Staphylococcus saprophyticus P1CEA4, a strain isolated from the porcine gastrointestinal tract. MALDI-TOF MS analysis revealed SapS variants corresponding to formylated and oxidized isoforms. To validate peptide identity, the active FPLC fraction was digested with trypsin and analyzed by LC-MS/MS. Proteome Discoverer 2.4 (Mascot search) initially provided 16% sequence coverage, while PEAKS Studio 10.5 improved coverage to 78%, identifying multiple peptides consistent with the predicted SapS sequence. These results provide strong proteomic evidence for SapS production in the native strain and support subsequent functional and structural characterization. The dataset contains processed outputs (Excel and HTML files) from Proteome Discoverer and PEAKS analysis, including peptide identifications, scores, probabilities, PTMs, and coverage statistics. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-08 |
| AnnouncementXML | Submission_2026-06-07_16:10:12.645.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD068573 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Concha Gil |
| SpeciesList | scientific name: Staphylococcus saprophyticus; NCBI TaxID: NEWT:29385; |
| ModificationList | acetylated residue; formylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-19 06:42:01 | ID requested | |
| ⏵ 1 | 2026-06-07 16:10:13 | announced | |
Publication List
| 10.1007/s12602-025-10823-9; |
| 10.6019/PXD068573; |
| Sevillano E, Lafuente I, Pe, ñ, a N, Cintas LM, Mu, ñ, oz-Atienza E, Hern, á, ndez PE, Borrero J, Saprophyticin S: A Novel Saposin-like, Leaderless Bacteriocin Produced by Staphylococcus saprophyticus P1CEA4 of Porcine Origin. Probiotics Antimicrob Proteins, 18(3):4940-4953(2026) [pubmed] |
Keyword List
| submitter keyword: Saprophyticin S |
| bacteriocin |
| leaderless bacteriocin |
| saposin-like fold |
| antimicrobial peptide |
| Staphylococcus saprophyticus |
| LC-MS/MS |
| MALDI-TOF |
| antimicrobial resistance |
Contact List
| Juan Borrero del Pino |
|---|
| contact affiliation | Department of Nutrition and Food Science, Faculty of Veterinary, Universidad Complutense de Madrid, Spain |
| contact email | jborrero@ucm.es |
| lab head | |
| Concha Gil |
|---|
| contact affiliation | UCM |
| contact email | cai.proteomica@pas.ucm.es |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD068573 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068573
- Label: PRIDE project
- Name: Proteomic validation of saprophyticin S (SapS), a novel leaderless bacteriocin from Staphylococcus saprophyticus P1CEA4
