PXD068528 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Global Proteomics to assess specificity of IAP recruiting TEAD1 degrader |
| Description | TEAD transcription factors (TEAD1-4) are the downstream most effectors of Hippo pathway, that controls organ development and tissue homeostasis. TEAD proteins form a transcriptional complex with co-activators YAP-TAZ to transcribe genes that regulate cell proliferation/apoptosis. Since Hippo pathway dysregulation is common in cancer phenotypes, inhibiting / degrading TEAD and its subsequent transcriptional activity is an attractive therapeutic strategy. We exploited targeted protein degradation approach using IAP proteins as E3 ligases to target TEAD1 and develop a potent isoform specific degrader. In this study we describe development and functional profiling of hits from three such series of IAP recruiting degraders (IPDs). We also report a detailed toolkit using structural, biophysical and cellular approaches to study IAP recruiting degraders that target the lipid pocket of TEAD proteins. To assess degradation and isoform specificity of our most potent hit XB2 A538, we also analysed global proteomic changes in mesothelioma model cell line NCI-H2052, treated for 16 hours with TEAD IPD A538, matched IAP negative control, TEAD negative control and DMSO, reported in data deposited here. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-30 |
| AnnouncementXML | Submission_2026-01-29_23:25:18.979.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Laura Dagley |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-19 01:32:10 | ID requested | |
| ⏵ 1 | 2026-01-29 23:25:19 | announced | |
Publication List
Keyword List
| submitter keyword: NCI-H2052 cells, Homo Sapiens,TEAD, Targeted protein degradation, Mesothelioma, Global proteomics, PROTAC, Hippo pathway, IAPs |
Contact List
| Prof. John Silke |
|---|
| contact affiliation | Laboratory Head & Head of Inflammation Division, Walter and Eliza Hall Institute of Medical Research (WEHI), Australia 3052 |
| contact email | silke@wehi.edu.au |
| lab head | |
| Laura Dagley |
|---|
| contact affiliation | WEHI |
| contact email | dagley.l@wehi.edu.au |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD068528 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068528
- Label: PRIDE project
- Name: Global Proteomics to assess specificity of IAP recruiting TEAD1 degrader
