PXD068527-1

PXD068527 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial RNA polymerase overexpression enhances transcription initiation and exercise capacity without increasing mature mtRNA levels
Description	POLRMT is the sole RNA polymerase in human mitochondria, where it generates primers for mitochondrial DNA (mtDNA) replication and transcribes the mtDNA to express genes encoding essential components of the oxidative phosphorylation (OXPHOS) system. Elevated POLRMT levels are found in several cancers and in mouse models with severe mitochondrial dysfunction. Here, we generated and characterized mice over-expressing Polrmt to investigate the physiological and molecular consequences of elevated POLRMT levels. Increasing POLRMT levels did not result in any pathological phenotype but led to increased exercise performance in male mice under stress conditions. Polrmt overexpression increased de novo transcription initiation, resulting in higher steady-state levels of the promoter-proximal L-strand transcript 7S RNA. Surprisingly, the abundance of mature mitochondrial RNAs was not affected by the elevated POLRMT levels. Furthermore, ubiquitous simultaneous overexpression of Polrmt and Lrpprc, which stabilizes mitochondrial messenger RNAs, did not increase steady-state levels of mitochondrial transcripts in the mouse. Our data show that POLRMT levels regulate transcription initiation, but additional regulatory steps downstream of transcription initiation and transcript stability limit OXPHOS biogenesis.
HostingRepository	PRIDE
AnnounceDate	2025-10-10
AnnouncementXML	Submission_2025-10-10_09:30:12.989.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilian Atanassov
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-19 01:30:33	ID requested
⏵ 1	2025-10-10 09:30:13	announced

Dataset with its publication pending

submitter keyword: POLRMT,mitochondria, label-free, DDA

Inge Kühl
contact affiliation	Cell Biology Department Institute of Integrative Biology of the Cell (I2BC,UMR9198) 1, Avenue de la Terrasse
contact email	inge.kuhl@i2bc.paris-saclay.fr
lab head
Ilian Atanassov
contact affiliation	Max Planck Institute for Biology of Aging
contact email	ilian.atanassov@age.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068527
     1. Label: PRIDE project
     2. Name: Mitochondrial RNA polymerase overexpression enhances transcription initiation and exercise capacity without increasing mature mtRNA levels