PXD068514-1

PXD068514 is an original dataset announced via ProteomeXchange.

Title	Deciphering the Anti-Biofilm Mechanism of Anthraquinone-2-Carboxylic Acid Against MRSA by Comprehensive Multi-Omics Analysis
Description	Background: Methicillin-resistant Staphylococcus aureus (MRSA) biofilms exhibit high levels of drug resistance and cause nosocomial infections in humans, thus posing a sustainable global health threat. This underscores the critical need to develop novel antibiofilm therapeutics with high efficacy and well-characterized mechanisms. In this study, we investigated the MRSA biofilm eradication mechanism of anthraquinone-2-carboxylic acid (ACA), a potent natural antibiofilm agent. Methods: The molecular mechanism underpinning the destruction of MRSA biofilm by ACA was systematically explored using integrated proteomic and metabolomic analysis. TMT-based proteomics quantified changes in protein expression, while complementary metabolomic data provided insight into associated pathway alterations. Transcript levels of key differentially expressed genes identified through omics analysis were validated by real-time quantitative polymerase chain reaction (RT-PCR), with intracellular reactive oxygen species (ROS) accumulation measured using the DCFH-DA method. The selected biofilm-associated biomarkers were rigorously validated using targeted parallel reaction monitoring (PRM) mass spectrometry. Conclusions: This study systematically elucidates the molecular mechanisms underlying the ACA-mediated disruption of MRSA biofilms, thereby establishing a robust theoretical framework for the rational design of ACA-based antibiofilm therapeutics. The identified pathways may provide novel targets for developing next-generation antibiofilm agents and pharmacodynamic biomarkers for MRSA biofilm treatment monitoring.
HostingRepository	iProX
AnnounceDate	2025-09-18
AnnouncementXML	Submission_2025-09-18_20:02:45.832.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	XiaoChun Shen
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-18 20:02:11	ID requested
⏵ 1	2025-09-18 20:02:46	announced

Dataset with its publication pending

submitter keyword: Anthraquinone-2-carboxylic acid, Methicillin-resistant Staphylococcus aureus, Antibiofilm, Proteomics and metabolomics

KaiLing Wang
contact affiliation	Dali University
contact email	kailingw@163.com
lab head
XiaoChun Shen
contact affiliation	Dali University
contact email	13618521641@163.com
dataset submitter

iProX dataset URI