PXD068513-1

PXD068513 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic analysis of PNUTS-associated proteins immunopurified with an anti-HA antibody
Description	Immunoprecipitated proteins on beads were washed once and resuspended in 50 mM ammonium bicarbonate, followed by the addition of 200 ng trypsin/Lys-C mix and an overnight incubation at 37ºC. The digests were reduced, alkylated, acidified, and desalted with GL-Tip SDB. Then, the eluates were evaporated and dissolved in 0.1% trifluoroacetic acid and 3% acetonitrile (ACN). The resultant peptides were analyzed by an EASY-nLC 1200 UHPLC connected to a Q Exactive Plus mass spectrometer in data-independent acquisition mode. Raw data were analyzed against an in silico spectral library generated from the UniProt database restricted to Drosophila melanogaster using DIA-NN 1.8.1. Missing values were imputed with random values sampled from the lower five percent of detected values.
HostingRepository	jPOST
AnnounceDate	2025-09-19
AnnouncementXML	Submission_2025-09-18_19:43:42.585.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hidetaka Kosako
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	L-methionine sulfoxide; Acetyl; S-carboxamidomethyl-L-cysteine
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-18 19:43:39	ID requested
⏵ 1	2025-09-18 19:43:42	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: PNUTS, anti-HA IP-MS

submitter keyword: PNUTS, anti-HA IP-MS

Contact List

Kensaku Murano
lab head
Hidetaka Kosako
contact affiliation	Tokushima University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004087/