PXD068503-1

PXD068503 is an original dataset announced via ProteomeXchange.

Title	Prenatal low-dose MeHg exposure leads to proteomic and transcriptomic alterations consistent with neurodegenerative disease in the cerebellum of C57BL/6 mice
Description	Methylmercury (MeHg) is a global pollutant that readily crosses the blood–brain barrier and placenta, posing significant risks to fetal neurodevelopment. While the cerebellum is a recognized target of MeHg toxicity in adults, the mechanisms underlying fetal exposure remain poorly defined. In this study, we investigated the neurotoxic effects of low-dose MeHg exposure (0.2 ppm via drinking water) on the cerebellums of prenatal C57BL/6 mice using integrated transcriptomic and proteomic analyses. Cerebellar tissues collected from postnatal day 90–120 (P90–120) mice (n = 3/group) were processed for RNA sequencing and proteomics analysis. Differentially expressed genes (DEGs) and proteins (DEPs) revealed significant changes in multiple pathways associated with neurodegeneration, including Huntington’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis. Overlapping transcriptomic and proteomic findings identified potential underlying mechanisms such as chemical carcinogenesis driven by reactive oxygen species and retrograde endocannabinoid signaling, underscoring the central role of oxidative stress in MeHg-induced neurotoxicity. Collectively, these results indicate that prenatal MeHg exposure induces persistent molecular alterations consistent with neurodegenerative processes and synaptic dysfunction, despite the absence of overt behavioral changes at the time of sacrifice. The long-term consequences for delayed symptom onset and the potential contribution of these changes to the etiology of neurodevelopmental disorders warrant further investigation.
HostingRepository	PRIDE
AnnounceDate	2026-02-06
AnnouncementXML	Submission_2026-02-06_15:05:21.646.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zoran Minic
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-18 16:29:26	ID requested
⏵ 1	2026-02-06 15:05:22	announced

10.2131/jts.51.89;

Loan A, D'Mello R, Li Y, Nurkan T, Minic Z, Wang J, Man Chan H, Prenatal low-dose MeHg exposure leads to proteomic and transcriptomic alterations consistent with neurodegenerative disease in the cerebellum of C57BL/6 mice. J Toxicol Sci, 51(2):89-100(2026) [pubmed]

submitter keyword: proteomics,MeHg, prenatal, disease latency, cerebellum, transcriptomics, neurodegenerative disease

Hing Man Chan
contact affiliation	Department of Biology, University of Ottawa, Ottawa, Canada, K1N 6N5
contact email	Laurie.Chan@uottawa.ca
lab head
Zoran Minic
contact affiliation	John L. Holmes Mass Spectrometry Facility, University of Ottawa
contact email	zminic@uottawa.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068503
     1. Label: PRIDE project
     2. Name: Prenatal low-dose MeHg exposure leads to proteomic and transcriptomic alterations consistent with neurodegenerative disease in the cerebellum of C57BL/6 mice