PXD068469-1

PXD068469 is an original dataset announced via ProteomeXchange.

Title	Bacterial outer membrane protein prevents enteric Salmonella infection
Description	Probiotics have become promising strategies for treating intestinal barrier dysfunction. However, the mechanisms by which probiotic bacteria interact with host cells to elicit beneficial effects remain unclear. Herein, we demonstrate that administration of E. coli Nissle 1917 (EcN) before Salmonella Typhimurium (STm) infection significantly suppressed STm pathogenesis in a mouse model. To address how bacterial interactions with intestinal epithelium affect STm infectivity, we developed a human gut-on-chip model coupled with computational fluid dynamics simulations, which recapitulated in vivo intestinal barrier protective phenotypes under physiologically relevant flow conditions. Using quantitative proteomics, we discovered that EcN-derived outer membrane protein MipA mediates protection by directly binding to intestinal epithelial integrin, inducing upregulation of integrin-linked kinase (ILK) to strengthen tight junctions and block STm invasion. Notably, expression of MipA in non-pathogenic STm conferred comparable barrier protection, establishing its pivotal role as a barrier enhancer. Our finding uncovers a novel mechanism whereby the bacterial membrane protein acts as a molecular trigger to enhance intestinal epithelial integrity, providing a significant framework for probiotic interventions against enteric infections.
HostingRepository	PRIDE
AnnounceDate	2026-03-10
AnnouncementXML	Submission_2026-03-10_06:15:46.441.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	huipai Peng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Escherichia coli; NCBI TaxID: NEWT:562; scientific name: Salmonella enterica subsp. enterica serovar Typhimurium str. STm1; NCBI TaxID: NEWT:1218143;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-17 21:59:33	ID requested
⏵ 1	2026-03-10 06:15:47	announced

Dataset with its publication pending

submitter keyword: host-microbe interaction, Salmonella Typhimurium, probiotics, gut-on chip,E. coli Nissle 1917

Nan Li
contact affiliation	CAS Key Laboratory for Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
contact email	nan.li@siat.ac.cn
lab head
huipai Peng
contact affiliation	CAS Key Laboratory for Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
contact email	hp.peng@siat.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD068469

PRIDE project URI

• PRIDE
  1. PXD068469
     1. Label: PRIDE project
     2. Name: Bacterial outer membrane protein prevents enteric Salmonella infection