⮝ Full datasets listing
PXD068464-1
PXD068464 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A role for the poly-asparagine repeat in the Plasmodium histone acetyltransferase, PfGCN5 |
| Description | Plasmodium falciparum possesses one of the most AT-rich genomes in nature (80.6%). A consequence is an asparagine-rich proteome. A quarter of P. falciparum proteins possess poly-asparagine repeats that can extend more than 100 residues. The role of these repeats has remained a mystery in the biology of this parasite. Here, we find that the poly-asparagine repeat-containing N terminus of the histone acetyltransferase PfGCN5 associates with the C-terminal catalytic domain after cleavage in the nucleus. Deletion of the repeat destabilizes the N-terminal polypeptide, leading to impaired parasite development and growth, particularly under stress conditions. Using high-resolution mass spectrometry and western blotting analysis, we uncovered a profound effect of the poly-asparagine repeat on acetylation of histones H3, H3.3, and H4. These findings suggest that the poly-asparagine repeat contributes to PfGCN5 acetyltransferase activity, a role previously attributed solely to its C-terminal domain. This report of a function for a poly-asparagine repeat in P. falciparum expands our understanding of a pervasive characteristic of its proteome. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-06-04 |
| AnnouncementXML | Submission_2026-06-04_09:27:23.960.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Daniel Goldberg |
| SpeciesList | scientific name: Plasmodium falciparum; common name: malaria parasite P. falciparum; NCBI TaxID: 5833; |
| ModificationList | Acetyl; Methyl; Trimethyl; Dimethyl |
| Instrument | Orbitrap Exploris 240 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-09-17 13:52:54 | ID requested | |
| ⏵ 1 | 2026-06-04 09:27:24 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Plasmodium, histone, PfGCN5, DatasetType:Proteomics |
Contact List
| Daniel Goldberg | |
|---|---|
| contact affiliation | Washington University in St. Louis |
| contact email | dgoldberg@wustl.edu |
| lab head | |
| Daniel Goldberg | |
| contact affiliation | Washington University in St. Louis |
| contact email | dgoldberg@wustl.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000099212/ |
