PXD068462 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of RNA chaperones by gradient profiling in the Lyme disease spirochete, Borrelia burgdorferi |
| Description | RNA-binding proteins (RBPs) play key roles in regulating gene expression in bacteria. However, relatively few RBPs have been discovered and characterized in Borrelia (Borreliella) burgdorferi, the causative agent of Lyme disease. We utilized gradient profiling to identify putative RBPs that co-sediment with small RNAs (sRNAs) and nascent mRNAs. We employed in vitro and in vivo assays to characterize the RNA chaperone activities of several proteins we identified in the gradient profiling. The previously hypothetical proteins BB0749, BB0713 and BB0796, as well as the chemotaxis-related protein CheY2 and the flagella-associated protein FlgV displayed dramatic RNA annealing and/or strand displacement activity. Moreover, in vivo Co-IP assays demonstrated BB0749 binds RNA in B. burgdorferi. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-29 |
| AnnouncementXML | Submission_2026-06-28_16:24:16.324.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD068462 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Christopher Ebmeier |
| SpeciesList | scientific name: Borreliella burgdorferi; NCBI TaxID: NEWT:139; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-17 11:08:46 | ID requested | |
| ⏵ 1 | 2026-06-28 16:24:17 | announced | |
Publication List
| 10.6019/PXD068462; |
| 10.1080/15476286.2026.2684395; |
| Van Gundy T, Schneider C, Ebmeier CC, Chambers GZ, Cramer NA, Chambers KM, Drecktrah D, Hyde JA, Skare JT, Marconi RT, Samuels DS, Lybecker MC, . RNA Biol, 23(1):1-23(2026) [pubmed] |
Keyword List
| submitter keyword: Lyme disease, Borrelia burgdorferi, RNA chaperone, RNA-binding protein |
Contact List
| Meghan Lybecker |
|---|
| contact affiliation | Center for Disease Control |
| contact email | oru7@cdc.gov |
| lab head | |
| Christopher Ebmeier |
|---|
| contact affiliation | University of Colorado Boulder |
| contact email | christopher.ebmeier@colorado.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068462
- Label: PRIDE project
- Name: Identification of RNA chaperones by gradient profiling in the Lyme disease spirochete, Borrelia burgdorferi
