PXD068425 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Deciphering Photosynthetic Protein Networks: A Crosslinking-MS Strategy for Studying Functional Thylakoid Membranes |
| Description | Photosynthesis relies on a dynamic network of protein-protein interactions that adapt to fluctuating light conditions. We present an XL-MS pipeline applied directly to functional thylakoid membranes from Arabidopsis thaliana and Spinacia oleracea. By combining PhoX crosslinker with the amphiphilic salt TMPAC, we captured reproducible structural insights into photosynthetic protein complexes while maintaining photosynthetic activity during crosslinking. The approach enables detection of hundreds of unique crosslinks within three days, expanding thylakoid protein interaction maps, and providing structural models of known and novel complexes. This study demonstrates the feasibility of probing functional photosynthetic membranes with XL-MS, paving the way for integrative analyses of dynamic organellar networks. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-14 |
| AnnouncementXML | Submission_2026-05-14_08:01:09.323.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Pascal Albanese |
| SpeciesList | scientific name: Spinacia sp.; NCBI TaxID: NEWT:29723; scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702; |
| ModificationList | acetylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480; Orbitrap Ascend |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-16 13:11:36 | ID requested | |
| ⏵ 1 | 2026-05-14 08:01:09 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Arabidopsis thaliana,Crosslinking-MS, Thylakoid membranes, Photosynthesis, Protein-protein interactions, Structural proteomics, Spinacia oleracea |
Contact List
| Pascal Albanese |
|---|
| contact affiliation | Light, Photosynthesis & Metabolism (LPM) & Exploring the Dynamics of Proteomes (EDyP) IRIG, CEA-Grenoble 17 rue des Martyrs, 38000 Grenoble, France |
| contact email | pascal.albanese@cea.fr |
| lab head | |
| Pascal Albanese |
|---|
| contact affiliation | Light, Photosynthesis & Metabolism (LPM) &
Exploring the Dynamics of Proteomes (EDyP)
IRIG, CEA-Grenoble
17 rue des Martyrs, 38000 Grenoble, France |
| contact email | pascal.albanese@cea.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068425
- Label: PRIDE project
- Name: Deciphering Photosynthetic Protein Networks: A Crosslinking-MS Strategy for Studying Functional Thylakoid Membranes
