PXD068423-1

PXD068423 is an original dataset announced via ProteomeXchange.

Title	Dual functionality of a small nucleolar RNA dictates the structure and function of translating ribosomes
Description	Leishmania causes cutaneous and visceral diseases affecting millions of patients worldwide. The parasite cycles between two different hosts, insect and mammalian. In this study, we determined the landscape of 2’-O- methylation (Nm) modification on Leishmania rRNA, and found two Nm positions that are differentially modified during cycling between the two life stages. Cryo-EM structures of ribosomes from cells overexpressing a guiding snoRNA compared to an inactive mutant at ~2.3-2.7 Å resolution did not reveal structural changes in the small subunit rRNA carrying the specific Nm modification. However, changes were observed in H68 of the large subunit rRNA due to a second base-pairing interaction, thus reviving the concept of chaperone activity for snoRNAs. Based on our high-resolution structure, translatome and tRNA analysis, we propose a mechanism whereby changes in the ribosome structure affected the release of specific tRNAs, which are correlated with changes in translation of only a subset of mRNAs.
HostingRepository	PRIDE
AnnounceDate	2026-06-02
AnnouncementXML	Submission_2026-06-02_02:48:04.831.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tirza Doniger
SpeciesList	scientific name: Leishmania major strain Friedlin; NCBI TaxID: NEWT:347515;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-16 11:49:43	ID requested
⏵ 1	2026-06-02 02:48:05	announced

Dataset with its publication pending

submitter keyword: cryo-EM,Leishmania, 2’-O-methylation, ribosome structure, RNA chaperone, C/D snoRNA

Shulamit Michaeli
contact affiliation	Institute of Nanotechnology and Advanced Materials Faculty of Life Sciences Bar-Ilan University
contact email	Shulamit.Michaeli@biu.ac.il
lab head
Tirza Doniger
contact affiliation	Bar-Ilan University
contact email	tirza.doniger@biu.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD068423

PRIDE project URI

• PRIDE
  1. PXD068423
     1. Label: PRIDE project
     2. Name: Dual functionality of a small nucleolar RNA dictates the structure and function of translating ribosomes