PXD068413-1

PXD068413 is an original dataset announced via ProteomeXchange.

Title	Atomic models of the conoid from the human parasite Toxoplasma gondii
Description	Apicomplexan parasites, responsible for toxoplasmosis, cryptosporidiosis, and malaria, invade host cells through a unique gliding motility mechanism powered by actomyosin motors and a dynamic organelle called the conoid. Using cryo-electron microscopy (cryo-EM) we determined structures of four essential complexes of the Toxoplasma gondii conoid: the preconoidal P2 ring, tubulin-based conoid fibers, and the subpellicular and intraconoidal microtubules. Our analysis identified 39 distinct conoid proteins, several of which are essential for parasite lytic growth, as revealed through genetic disruption studies. Comparative analysis of the tubulin-containing complexes sheds light on their functional specialization by microtubule-associating proteins, while the structure of the preconoidal ring pinpoints the site of actin polymerization and initial translocation, enhancing our mechanistic understanding of gliding motility and therefore parasite invasion
HostingRepository	PRIDE
AnnounceDate	2025-11-14
AnnouncementXML	Submission_2025-11-14_06:43:55.368.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD068413
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sophe Alvarez
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: NEWT:5811;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-16 07:39:18	ID requested
⏵ 1	2025-11-14 06:43:55	announced

10.1038/S41594-025-01728-W;

10.6019/PXD068413;

submitter keyword: Toxoplasma, parasite, human, conoid protein

Sophie Alvarez
contact affiliation	Proteomics and Metabolomics Facility, University of Nebraska-Lincoln
contact email	salvarez@unl.edu
lab head
Sophe Alvarez
contact affiliation	University of Nebraska - Lincoln
contact email	salvarez@unl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD068413

PRIDE project URI

• PRIDE
  1. PXD068413
     1. Label: PRIDE project
     2. Name: Atomic models of the conoid from the human parasite Toxoplasma gondii