PXD068407 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mass spectrometry-based validation of BPTF knockout in human induced pluripotent stem cells |
| Description | We generated a BPTF knockout human induced pluripotent stem cell (iPSC) line using CRISPR-Cas9 and confirmed loss of the targeted locus by genotyping. Quantitative mass spectrometry verified complete absence of BPTF protein in the knockout clone. This validated line was used for downstream functional assays in the somitoid system, a 3D iPSC-derived model that recapitulates early human somite development. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-07 |
| AnnouncementXML | Submission_2026-08-07_03:02:43.411.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | S Stelloo |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-16 03:58:41 | ID requested | |
| ⏵ 1 | 2026-08-07 03:02:44 | announced | |
Publication List
| 10.1016/j.stemcr.2026.103023; |
| Alejo-Vinogradova MT, Str, ø, m YE, Lamers LA, Chang YW, Hendriksen MJ, Moosajee M, Vermeulen M, Stelloo S, From stem cells to somites: Revealing genetic and exogenous factors of human embryogenesis. Stem Cell Reports, ():103023(2026) [pubmed] |
Keyword List
| submitter keyword: stem cells, BPTF, knockout, ipscs |
Contact List
| Michiel vermeulen |
|---|
| contact affiliation | Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, 6525 GA Nijmegen, The Netherlands |
| contact email | michiel.vermeulen@ru.nl |
| lab head | |
| S Stelloo |
|---|
| contact affiliation | Department of Urology, Radboud University Medical Center (RUMC), Nijmegen, The Netherlands |
| contact email | stelloo@science.ru.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD068407 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068407
- Label: PRIDE project
- Name: Mass spectrometry-based validation of BPTF knockout in human induced pluripotent stem cells
