⮝ Full datasets listing
PXD068386-1
PXD068386 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Capturing the RNA-binding proteome from plants using orthogonal organic phase separation |
| Description | Summary RNA-binding proteins (RBPs) regulate many processes related to RNA biogenesis, localization, half-life and function. Here, we present a protocol for the en masse isolation of RBPs cross-linked to RNA and provide a strategy for proteomics analysis. We describe the steps for in vivo UV-crosslinking of RNA-protein complexes, tissue lysis, fractionation and purification of crosslinked RNA-protein adducts using organic solvents. Although the protocol was developed for Nicotiana benthamiana leaves, it can be optimized for use in different plants and tissues. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-11-04 |
| AnnouncementXML | Submission_2025-11-04_03:42:19.205.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Gertjan Kramer |
| SpeciesList | scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; |
| ModificationList | Oxidation; Carbamidomethyl |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-09-15 08:02:21 | ID requested | |
| ⏵ 1 | 2025-11-04 03:42:19 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: RNA binding proteins, Proteomics, OOPS, XRNAX, Plant Proteomics, Plant Science, DatasetType:Proteomics |
Contact List
| Gertjan | |
|---|---|
| contact affiliation | laboratory for Mass Spectrometry of Biomolecules, University of Amsterdam |
| contact email | gertjan.kramer@uva.nl |
| lab head | |
| Gertjan Kramer | |
| contact affiliation | Universiteit van Amsterdam |
| contact email | gertjan.kramer@uva.nl |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000099169/ |
