⮝ Full datasets listing
PXD068383-1
PXD068383 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | An atlas from mass spectrometry screening of HDAC inhibitors revealed increased histone H2A. Z acetylation in breast cancer |
| Description | Histone deacetylase inhibitors (HDACi) have emerged as valuable therapeutics in cancer and other diseases, yet their effect on histone post-translational modification remains incompletely characterized. Here, we applied quantitative mass spectrometry and high throughput sequencing to systematically profile site-specific histone modification changes in response to a panel of HDAC inhibitors. This platform enabled mapping of histone modification changes across hundreds of sites including low abundant histone marks. Furthermore, integrative analysis of ChIP-seq and RNA-seq identified genome wide binding site for a low-abundant histone modification H2A.Z acetylation in HeLa cells and MDA-MB-231 breast cancer cells, highlighting the role of H2A.Z acetylation in regulating gene expression through various biological pathways and specific genes involved in tumor suppressor pathways. Our findings provide a functional resource for identification and quantification of epigenetic changes and transcriptional regulation of histone H2A.Z acetylation upon pharmacological perturbation |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-03-18 |
| AnnouncementXML | Submission_2026-03-18_08:47:53.942.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rashmi Karki |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Acetyl; Methyl |
| Instrument | Orbitrap Exploris 240 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-09-15 07:53:29 | ID requested | |
| ⏵ 1 | 2026-03-18 08:47:54 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: histone deacetylase inhibitors, posttranslational modification, histone H2A.Z acetylation, mass spectrometry, DatasetType:Proteomics |
Contact List
| Benjamin A. Garcia | |
|---|---|
| contact affiliation | Washington University in St. Louis |
| contact email | bagarcia@wustl.edu |
| lab head | |
| Rashmi Karki | |
| contact affiliation | Washington University in St louis |
| contact email | karkir@wustl.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000099168/ |
