PXD068376 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Structural basis of gate dynamics at the outer membrane lipopolysaccharide holo-translocon |
| Description | Gram-negative bacteria resist many harmful chemicals, protected by an asymmetric outer membrane (OM) containing lipopolysaccharide (LPS) in its external leaflet. Leaflet-selective assembly of LPS is an essential, yet mechanistically elusive process. This reaction is mediated by the OM LPS core translocon, composed of the transmembrane protein LptD and its cognate lipoprotein LptE. Here, we characterize two additional translocon subunits, the lipoproteins LptM and YedD, uncovering their impact on LptD conformational dynamics. This dataset includes native MS allowing the identification of YedD, after gas-phase dissociation from the LptDE/LptDEM complexes (complex-down MS). We also include here bottom-up proteomics data after trypsin digestion from SDS-PAGE gel bands, confirming the nature of YedD in the purified complex. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-01 |
| AnnouncementXML | Submission_2025-11-30_16:11:20.724.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Julien Marcoux |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: NEWT:562; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive; Synapt MS |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-15 04:51:07 | ID requested | |
| ⏵ 1 | 2025-11-30 16:11:21 | announced | |
Publication List
| 10.1038/s41467-025-65370-2; |
| Chen H, Siroy A, Morales V, Gurvi, č D, Quentin Y, Balor S, Abuta'a YA, Marteau M, Froment C, Caumont-Sarcos A, Marcoux J, Stansfeld PJ, Fronzes R, Ieva R, Structural basis of lipopolysaccharide assembly by the outer membrane translocon holo-complex. Nat Commun, 16(1):10404(2025) [pubmed] |
Keyword List
| submitter keyword: LPS translocon,native MS, gel digestion |
Contact List
| Julien Marcoux |
|---|
| contact affiliation | ProteoToul, Institute of Pharmacology and Structural Biology (IPBS), CNRS, Toulouse |
| contact email | julien.marcoux@ipbs.fr |
| lab head | |
| Julien Marcoux |
|---|
| contact affiliation | IPBS |
| contact email | julien.marcoux@ipbs.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068376
- Label: PRIDE project
- Name: Structural basis of gate dynamics at the outer membrane lipopolysaccharide holo-translocon
