PXD068329 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Optimizing Mass Spectrometry Methods to Uncover M. tuberculosis Biomarkers in Extracellular Vesicles |
| Description | Individuals exposed to Mtb can experience a range of outcomes: early bacterial clearance, latent Mtb infection (LTBI), asymptomatic TB (bacteriologically confirmed or not), or symptomatic TB (bacteriologically confirmed or clinically diagnosed). Current tools cannot reliably predict or distinguish these stages. Previous studies have identified Mtb peptides in extracellular vesicles (EVs) from TB patients’ serum, highlighting their potential as biomarkers. We aimed to identify Mtb proteins and peptides in serum EVs as potential biomarkers across TB stages, with a focus on differentiating asymptomatic individuals. We evaluated multiple mass spectrometry approaches, including optimizing the liquid chromatography gradient (more hydrophobic), and iteratively removed dominant peptides, to identify and differentially quantify Mtb peptides. We analyzed serum samples from healthy, HIV-negative South African adult volunteers enrolled in a TB risk study. Samples were categorized as prevalent TB (diagnosed within 30 days of baseline), incident TB (collected at baseline from participants who later developed TB), activated TB (collected at diagnosis, during follow-up, from incident cases), or controls (collected at baseline from participants who did not develop TB within the 15 months of the study). |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-03 |
| AnnouncementXML | Submission_2026-03-03_12:07:22.582.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD068329 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Luisa Nieto Ramirez |
| SpeciesList | scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: NEWT:83332; |
| ModificationList | acetylated residue; monohydroxylated residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-12 09:42:58 | ID requested | |
| ⏵ 1 | 2026-03-03 12:07:23 | announced | |
Publication List
Keyword List
| submitter keyword: asymptomatic, proteomics, subclinical TB, discovery,Serum, mycobacterium |
Contact List
| Karen Dobos |
|---|
| contact affiliation | Professor, Microbiology, Immunology, and Pathology Mycobacteria Research Laboratories Scientific Director, Rocky Mountain Regional Biocontainment Laboratory Editor-in-chief, BMC Mycobacteria |
| contact email | Karen.Dobos@ColoState.edu |
| lab head | |
| Luisa Nieto Ramirez |
|---|
| contact affiliation | Colorado State University |
| contact email | luisam@colostate.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD068329 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068329
- Label: PRIDE project
- Name: Optimizing Mass Spectrometry Methods to Uncover M. tuberculosis Biomarkers in Extracellular Vesicles
