PXD068314-1

PXD068314 is an original dataset announced via ProteomeXchange.

Title	Distinct steps of nuclear maturation of human pre-60S complexes require the activity of GTPases, including GNL3L
Description	Production of the eukaryotic ribosomal subunits (40S and 60S) is a highly dynamic process in which numerous assembly factors coordinate structural rearrangements of pre-ribosomal complexes to achieve their mature, functional architectures. Across the domains of life, GTPases leverage their functions as molecular switches to induce conformational changes that drive key steps in subunit maturation. Three GTPases, GTPBP4, GNL2 and GNL3 have been detected in nucleolar/nucleoplasmic human pre-60S complexes. Here we compositionally analyze the pre-ribosomal particles associated with each of these GTPases and demonstrate the requirement of these enzymes, and their abilities to bind and hydrolyze GTP, for distinct steps in pre-ribosomal RNA processing. We further reveal that the GNL3 paralogue, GNL3L, also associates with pre-ribosomes, and we map RNA contact sites on GNL3L as well as GNL3L binding sites on pre-rRNAs. Lack of GNL3L impairs synthesis of the 60S rRNAs and expression of GTPase inactive GNL3L causes defects in early steps of pre-rRNA processing. Impaired GTP hydrolysis by GNL3L leads to its accumulation on pre-60S particles, together with other assembly factors with proximal binding sites. Our data further demonstrate that the GTPase activity of GNL3L is required for maintaining 60S subunit levels, protein synthesis and cellular proliferation.
HostingRepository	PRIDE
AnnounceDate	2026-03-09
AnnouncementXML	Submission_2026-03-09_04:00:49.016.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Lenz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-12 04:53:06	ID requested
⏵ 1	2026-03-09 04:00:50	announced

Thom, é CC, Lemus-Diaz N, Bloch von Blottnitz KI, Tagn, è, res S, Klein Helmkamp M, Honemann-Capito M, Hackert P, Moshkovskii S, Lenz C, Bohnsack MT, Urlaub H, Bohnsack KE, Distinct steps of nuclear maturation of human pre-60S complexes require the activity of GTPases including GNL3L. Nucleic Acids Res, 54(5):(2026) [pubmed]

10.1093/nar/gkag154;

submitter keyword: Ribosome biogenesis, GTPase, ribosomal protein, GTPBP4, GNL2, pre-rRNA processing, GNL3, translation

Christof Lenz
contact affiliation	Core Facility Proteomics, Department of Clinical Chemistry, University Medical Center Goettingen, Germany
contact email	christof.lenz@med.uni-goettingen.de
lab head
Christof Lenz
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	christof.lenz@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD068314

PRIDE project URI

• PRIDE
  1. PXD068314
     1. Label: PRIDE project
     2. Name: Distinct steps of nuclear maturation of human pre-60S complexes require the activity of GTPases, including GNL3L