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PXD068295-1
PXD068295 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitative protein analysis and glycan profiling of umbilical cord plasma and corresponding maternal plasma samples |
| Description | Protein glycosylation is an essential post-translational modification boosting proteomic diversity. The major groups of human plasma glycoproteins are immunoglobulins (Igs) and acute phase proteins (APPs). Changes in plasma Igs and APPs levels and their glycosylation have been previously associated with inflammatory diseases, several types of cancer, pregnancy, or aging. In this study, we present a novel approach to investigate the neonatal and maternal plasma at the end of pregnancy using a combination of targeted quantitative proteomics and total plasma N-glycome profiling. We present a new SRM-based multiplex assay to quantify 19 APPs and 7 Igs. We applied this assay to 98 samples of umbilical cord plasma and corresponding maternal plasma samples and observed significantly lower levels of all analyzed proteins in neonates, apart from the glycoprotein alpha-2-macroglobulin. Unlike previous methods, our SRM-MS assay allows us to quantify dozens of proteins simultaneously and to distinguish protein variants. We compared maternal and fetal total plasma-released N-glycomes in the same set of samples. We quantified 66 individual glycans, which were used to calculate 73 glycosylation traits. In neonates, the major N-glycans were di-antennary complex-type with core fucosylation, and the production of higher, branched, sialylated N-glycans was limited. |
| HostingRepository | PanoramaPublic |
| AnnounceDate | 2025-09-15 |
| AnnouncementXML | Submission_2025-09-15_16:51:44.026.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Zdenek Spacil |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4) |
| Instrument | 6495B Triple Quadrupole LC/MS |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-09-11 14:19:11 | ID requested | |
| ⏵ 1 | 2025-09-15 16:51:44 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: glycosylation, immunoglobulins, acute-phase proteins, N-glycans, targeted proteomics, mass spectrometry, pregnancy, neonates |
Contact List
| Zdenek Spacil | |
|---|---|
| contact affiliation | Masaryk University, Faculty of Science, RECETOX |
| contact email | spacil@recetox.muni.cz |
| lab head | |
| Zdenek Spacil | |
| contact affiliation | Masaryk University, Faculty of Science, RECETOX |
| contact email | spacil@recetox.muni.cz |
| dataset submitter | |
Full Dataset Link List
| Panorama Public dataset URI |
