PXD068275 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Complete structures of the YenTc holotoxin prepore and pore reveal the evolutionary basis for chitinase incorporation into ABC toxins |
| Description | ABC toxins are toxin-translocating, pore-forming proteins found in a wide range of insecticidal bacteria and some mammalian pathogens. The Yersinia entomopahaga toxin complex (YenTc) belongs to a distinct subclass of ABC toxins, defined by a divergent molecular architecture. Structural details that define their mechanism of action remain to be elucidated. Here we determine structures of the YenTc holotoxin assembly in both prepore and pore-forming configurations using cryo-EM in conjunction with Alphafold2-assisted structural modelling of flexible domains. We define the structural mechanism via which enzymatically-active chitinase subunits are incorporated, and show using phylogenetic analyses that this subclass-defining feature has evolved relatively recently. Our structures point to the existence of distinct conformational states in YenTc, which may distinguish it from other structurally-characterised ABC toxins, or represent novel states on a shared mechanistic trajectory. Thus, our findings enhance our understanding of the structural diversity that defines distinct ABC toxin subclasses. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-22 |
| AnnouncementXML | Submission_2025-12-21_17:02:00.998.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jason Low |
| SpeciesList | scientific name: Yersinia entomophaga; NCBI TaxID: NEWT:935293; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-10 23:39:30 | ID requested | |
| ⏵ 1 | 2025-12-21 17:02:01 | announced | |
Publication List
| 10.1038/s41467-025-66050-x; |
| Low YS, Roche SG, Aleksandrova NA, Foley G, Low JK, Box JK, Croll TI, Chassagnon IR, Lott JS, Deplazes E, Bod, é, n M, Hurst MR, Piper SJ, Landsberg MJ, Complete structures of the YenTc holotoxin prepore and pore reveal the evolutionary basis for chitinase incorporation into ABC toxins. Nat Commun, 16(1):11121(2025) [pubmed] |
Keyword List
| submitter keyword: YenTc, XLMS, pores,ABC toxins, Alphafold, cryoEM |
Contact List
| Michael J Landsberg |
|---|
| contact affiliation | School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia Queensland 4072, Australia ARC Industry Transformation Training Centre for Cryo-electron Microscopy of Membrane Proteins Institute for Molecular Bioscience, The University of Queensland, St Lucia Queensland 4072, Australia |
| contact email | m.landsberg1@uq.edu.au |
| lab head | |
| Jason Low |
|---|
| contact affiliation | The University of Sydney |
| contact email | jason.low@sydney.edu.au |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD068275 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068275
- Label: PRIDE project
- Name: Complete structures of the YenTc holotoxin prepore and pore reveal the evolutionary basis for chitinase incorporation into ABC toxins
