PXD068257-1

PXD068257 is an original dataset announced via ProteomeXchange.

Title	Antigen-specific IgG Glycosylation Profiles in Hamsters and Macaques Following COVID-19 Vaccination
Description	Vaccination triggers the production of antigen-specific antibodies, including IgG. IgG molecules are glycosylated at the Fc region, and these glycan modifications markedly influence Fc receptor binding and downstream immune functions. Notably, infections with enveloped viruses such as SARS-CoV-2 can trigger the production of afucosylated IgG, which enhances FcγRIIIa binding and promotes antibody-dependent cellular cytotoxicity. Despite its importance, the glycosylation profiles of antigen-specific IgG following vaccination remain understudied, particularly in animal models. In this study, we investigated the Fc glycosylation patterns of antigen-specific IgG in hamsters and rhesus macaques following immunization with the Ad26.COV2.S COVID-19 vaccine. Overall, our findings demonstrate that IgG Fc glycosylation dynamics in these animal models largely parallel those in humans. For example, we observed a transient afucosylated IgG response in both species, resembling the response previously reported in humans. These results indicate that IgG Fc glycosylation responses to vaccination in macaques and hamsters recapitulate key features of the human response, supporting their use as translational models for adenovirus vector-based vaccination studies.
HostingRepository	PRIDE
AnnounceDate	2026-03-11
AnnouncementXML	Submission_2026-03-11_07:21:15.390.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bart Claushuis
SpeciesList	scientific name: Mesocricetus auratus (Golden hamster); NCBI TaxID: NEWT:10036; scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: NEWT:9544;
ModificationList	No PTMs are included in the dataset
Instrument	impact HD

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-10 09:09:41	ID requested
⏵ 1	2026-03-11 07:21:15	announced

Dataset with its publication pending

submitter keyword: glycosylation,antibody, SARS-COV-2, vaccination, COVID-19

Manfred Wuhrer
contact affiliation	Center for Proteomics and Metabolomics, Leiden University Medical Center
contact email	m.wuhrer@lumc.nl
lab head
Bart Claushuis
contact affiliation	Leiden University Medical Center
contact email	b.claushuis@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD068257

PRIDE project URI

• PRIDE
  1. PXD068257
     1. Label: PRIDE project
     2. Name: Antigen-specific IgG Glycosylation Profiles in Hamsters and Macaques Following COVID-19 Vaccination