PXD068252-1

PXD068252 is an original dataset announced via ProteomeXchange.

Title	Acute and Chronic Effects of GLP-1 Receptor Agonist Liraglutide on the Sprague Dawley Rat Neurointermediate Lobe Proteome and Phosphoproteome
Description	Obesity and its co-morbidities, such as diabetes and hypertension, can significantly reduce a person’s quality of life and place huge pressure on healthcare resources. When we eat a meal our gut and brain release hormones to control the amount of food and fluid we ingest to prevent overeating. One of these hormones is called glucagon-like peptide 1 (GLP-1) and is released from intestinal cells in response to food intake but also produced and released in the brain. Drug analogues of GLP-1 are already successfully in use in the clinic to treat both diabetes and obesity. The aim of this work was to extend our earlier phosphoproteomic findings about interactions of GLP-1 receptor agonist liraglutide, a drug that is approved for diabetes and obesity treatment in humans, with the neurointermediate lobe (posterior pituitary + intermediate lobe). This work focussed on the proteome and phosphoproteome of the neurointermediate lobe of the male and female rat pituitary gland. Liraglutide (Tocris, 6517/1) was prepared in normal saline to a concentration of 100 μg/ml. Injections of 100 μg/kg body weight or normal saline vehicle were performed intraperitoneally using a 30-gauge insulin syringe. Acute study - Animals were housed singly for 4 weeks before experimentation. Injections were performed during the final 30 minutes of the light phase before the lights went off. Animals were killed 4-5 hours (n = 6 animals per group) after injection of liraglutide or vehicle. Chronic study - Animals were singly housed for 2 weeks prior to experimentation. Injections were performed daily for 14 days during the final 30 minutes of the light phase before the lights went off. Animals were killed 4-5 hours (n = 6 animals per group) after the final injection of liraglutide or vehicle.
HostingRepository	PRIDE
AnnounceDate	2026-06-26
AnnouncementXML	Submission_2026-06-26_05:37:17.088.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Greenwood
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-10 04:34:41	ID requested
⏵ 1	2026-06-26 05:37:17	announced

Dataset with its publication pending

submitter keyword: Phosphoproteomics, Liraglutide, Pituitary gland, Posterior pituitary,Intermediate lobe, Phosphorylation

Michael Paul Greenwood
contact affiliation	School of Physiology, Pharmacology and Neuroscience University of Bristol Biomedical Sciences Building University Walk Bristol - BS8 1TDE United Kingdom
contact email	mike.greenwood@bristol.ac.uk
lab head
Michael Greenwood
contact affiliation	University of Bristol
contact email	mike.greenwood@bristol.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068252
     1. Label: PRIDE project
     2. Name: Acute and Chronic Effects of GLP-1 Receptor Agonist Liraglutide on the Sprague Dawley Rat Neurointermediate Lobe Proteome and Phosphoproteome