PXD068228 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Evolutionarily Convergent Mechanism of Formin Regulation Coordinates Actin Polymerization in Apicomplexan Parasites |
| Description | Apicomplexan parasites rely on actin-based motility for host cell invasion and for their dissemination. Although previous studies implicate formin 1 (FRM1) in governing this process in Toxoplasma, the mechanisms that position it at the cell apex and regulate its activity during the transition from intracellular replication to active motility remain unclear. Here, we demonstrate that FRM1 is complexed with a protein methyl transferase that stabilizes it from degradation and positions it at the cell apex. Moreover, we identify a conserved intramolecular interaction within FRM1 that serves as a critical regulatory switch that is functionally conserved in Plasmodium and other apicomplexan parasites. Using structural modeling combined with biochemical assays, we define two regulatory elements located upstream of the FH2 domain that mediate autoinhibition. Repositioning these elements by activation of an intramolecular switch relieves autoinhibition and activates actin polymerization by the FH2 domain. Collectively, these findings elucidate how precise regulation of FRM1 coordinates actin polymerization, parasite motility and host cell invasion. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-04 |
| AnnouncementXML | Submission_2025-11-04_07:47:21.199.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD068228 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Sophe Alvarez |
| SpeciesList | scientific name: Toxoplasma gondii ME49; NCBI TaxID: NEWT:508771; |
| ModificationList | N6; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-09 08:32:54 | ID requested | |
| ⏵ 1 | 2025-11-04 07:47:21 | announced | |
Publication List
Keyword List
| submitter keyword: methylation, gliding motility, intramolecular regulation, autoinhibition, actin polymerization,Formin |
Contact List
| Michael Naldrett |
|---|
| contact affiliation | Proteomics and Metabolomics Facility, University of Nebraska- Lincoln |
| contact email | mnaldrett@unl.edu |
| lab head | |
| Sophe Alvarez |
|---|
| contact affiliation | University of Nebraska - Lincoln |
| contact email | salvarez@unl.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068228
- Label: PRIDE project
- Name: Evolutionarily Convergent Mechanism of Formin Regulation Coordinates Actin Polymerization in Apicomplexan Parasites
