PXD068185 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Muscle-specific increased expression of JAG1 improves the skeletal muscle phenotype in dystrophin-deficient mice |
| Description | Therapeutic strategies for Duchenne muscular dystrophy (DMD) will likely require complementary approaches. One possibility is to explore genetic modifiers that improve muscle regeneration and function. The beneficial effects of the overexpression of Jagged-1 were described in escaper golden retriever muscular dystrophy (GRMD) dogs that had a near-normal life and validated in dystrophin-deficient zebrafish. To clarify the underlying biology of JAG1 overexpression in dystrophic muscles, we generated a transgenic mouse (mdx5cv-JAG1) model that lacks dystrophin and overexpresses human JAG1 in striated muscles. Skeletal muscles from mdx5cv-JAG1 and mdx5cv mice were studied at 1-, 4-, and 12-mo time points. JAG1 expression in mdx5cv-JAG1 increased by 3 to 5 times compared to mdx5cv. Consequently, mdx5cv-JAG1 muscles were significantly bigger and stronger than dystrophic controls, along with an increased number of myofibers. Proteomics data show increased dysferlin in mdx5cv-JAG1 muscles and an association of the histone methyltransferase Nsd1 with the phenotype. Our data support the positive effect of JAG1 overexpression in dystrophic muscles. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-30 |
| AnnouncementXML | Submission_2026-03-29_16:20:07.903.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Joao Paulo |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | monohydroxylated residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-08 09:16:22 | ID requested | |
| ⏵ 1 | 2026-03-29 16:20:08 | announced | |
Publication List
| 10.1073/pnas.2506437122; |
| de Souza Leite F, Lambert MR, Zhang TY, Conner JR, Paulo JA, Oliveira SF, Guha Thakurta S, Bowles J, Gussoni E, Gygi SP, Widrick JJ, Kunkel LM, improves the skeletal muscle phenotype in dystrophin-deficient mice. Proc Natl Acad Sci U S A, 122(39):e2506437122(2025) [pubmed] |
Keyword List
| submitter keyword: Duchenne muscular dystrophy; Jagged-1; mouse model; genetic modifier; physiology |
Contact List
| Louis M. Kunkel |
|---|
| contact affiliation | Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA 02115, USA Department of Genetics and Pediatrics, Harvard Medical School, Boston, MA 02115, USA |
| contact email | kunkel@enders.tch.harvard.edu |
| lab head | |
| Joao Paulo |
|---|
| contact affiliation | Harvard Medical School |
| contact email | joao_paulo@post.harvard.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068185
- Label: PRIDE project
- Name: Muscle-specific increased expression of JAG1 improves the skeletal muscle phenotype in dystrophin-deficient mice
