PXD068165-1

PXD068165 is an original dataset announced via ProteomeXchange.

Title	DARTS proteomic response of HepG2 cells to a-linolenic acid
Description	HepG2 cells were washed twice with pre-cooled PBS and added PBS with 1% protease inhibitors. Cellular proteins were extracted through five cycles of freeze-thaw lysis in liquid nitrogen (5 min freezing) followed by 37 ℃ thawing and the lysates were clarified by centrifugation at 12,000 rpm for 15 min at 4°C. The resulting supernatants were collected for total proteins quantification and the bicinchoninic acid (BCA) assay. A 50 μL reaction system containing 200 μg total protein was aliquoted for further proteomics analysis. Experimental groups received either ALA (treatment) or BSA (control) at a final concentration of 100 μM (n=3 replicates per group). Following thorough mixing, samples were incubated at 37°C for 30 min. Pronase was then added at 0.5% (w/w relative to total protein mass) and incubation continued for an additional 30 min at 37°C. Protein samples (±pronase) were mixed with 5× SDS-PAGE loading buffer and denatured by boiling for 5 min, followed by separation via polyacrylamide gel electrophoresi
HostingRepository	iProX
AnnounceDate	2025-09-08
AnnouncementXML	Submission_2025-09-08_01:34:22.520.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shiyu Yin
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-08 01:28:49	ID requested
⏵ 1	2025-09-08 01:34:22	announced

Dataset with its publication pending

submitter keyword: DARTS a-linolenic acid

Zewen Wen
contact affiliation	Shenzhen Nanshan People's Hospital
contact email	wenzw05@163.com
lab head
Shiyu Yin
contact affiliation	Shenzhen Nanshan People's Hospital
contact email	ysy7772023@163.com
dataset submitter

iProX dataset URI