PXD068150-1

PXD068150 is an original dataset announced via ProteomeXchange.

Title	Phospho-proteome profiling in human induced pluripotent stem cells and derived human neurons reveals targets of TBK1 in ALS/FTD-1 associated autophagy networks
Description	TBK1 (TANK-binding kinase 1) is a ubiquitously expressed IKK-related kinase implicated in diverse cellular processes, including interferon signaling, apoptosis, and autophagy. Loss-of-function variants in TBK1 are strongly associated with amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). However, the targets of TBK1 in neurons are unknown, and how haploinsufficiency for TBK1 leads to age-related neurodegeneration remains unresolved. Here, we utilized sets of isogenic induced pluripotent stem cells (iiPSCs) for unbiased, quantitative phospho-proteomics across tens of thousands of phospho-sites, normalized to protein abundance, in proliferating stem cells and induced excitatory neurons. We found that TBK1 primarily regulates the phosphorylation of selective autophagy proteins, particularly phospho-serine residues within cargo receptors, and endo-lysosomal pathways.
HostingRepository	PRIDE
AnnounceDate	2025-09-11
AnnouncementXML	Submission_2025-09-11_15:06:24.573.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Oses-Prieto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse; Orbitrap Fusion Lumos; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-07 22:17:39	ID requested
⏵ 1	2025-09-11 15:06:24	announced

Dataset with its publication pending

submitter keyword: autophagy, neurons,TNK1, phosphorylation

Daniel Mordes
contact affiliation	Department of Pathology, University of California, San Francisco. San Francisco, CA, USA. Institute for Neurodegenerative Diseases, Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, CA, USA.
contact email	daniel.mordes@ucsf.edu
lab head
Juan Oses-Prieto
contact affiliation	UCSF
contact email	joses@cgl.ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD068150

PRIDE project URI

• PRIDE
  1. PXD068150
     1. Label: PRIDE project
     2. Name: Phospho-proteome profiling in human induced pluripotent stem cells and derived human neurons reveals targets of TBK1 in ALS/FTD-1 associated autophagy networks