⮝ Full datasets listing
PXD068094-1
PXD068094 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | IsoPS-DIA: Dual Functionality of Absolute Targeted Quantification and Global Proteome Profiling |
| Description | In this project, we developed a dual-functional data acquisition method, IsoPS-DIA, which incorporates two complementary designs: target windows and a non-target window. First, we evaluated the sensitivity and quantitative performance of targeted peptides, as well as the global proteomic profiling capabilities of IsoPS-DIA, in comparison with conventional fixed-window DIA (Fix-DIA), variable-window DIA based on precursor density (Var-DIA), and PRM. These comparisons were conducted using normal mouse lung tissue peptide samples spiked with targeted peptides, resulting in a total of 14 MS raw files and one searched result for each MS method. Next, IsoPS-DIA was applied to analyze six NSCLC cell lines—A549, PC9, H1975, H3255, CL68, and CL97—each harboring a distinct EGFR mutation (Wild type, Del19, L858R/T790M, L858R, Del19/T790M, and G719A, respectively). Samples were digested with Lys-C, and each cell line was analyzed in triplicate, yielding a total of 18 MS raw files and one Spectronaut searched result. Additionally, to generate Del19 peptides, Glu-C digestion was performed on PC9 and CL68, resulting in six more MS raw files. We also provide the result of IsoPS-DIA performed in Q-TOF instruments (40 MS raw files). In total, 124 raw files and their corresponding searched results were uploaded. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-08-11 |
| AnnouncementXML | Submission_2026-08-11_00:03:02.754.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Huan-Chi Chiu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | L-cysteine methyl disulfide; L-methionine sulfoxide; alpha-amino acetylated residue; deamidated L-glutamine; deamidated L-asparagine |
| Instrument | Orbitrap Eclipse; Orbitrap Fusion Lumos; instrument; TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-09-04 14:46:51 | ID requested | |
| ⏵ 1 | 2026-08-11 00:03:03 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Data-independent acquisition mass spectrometry, Quantitative proteomics, Targeted proteomics, Protein variant |
Contact List
| Yu-Ju Chen | |
|---|---|
| lab head | |
| Huan-Chi Chiu | |
| contact affiliation | IoC-Academia Sinica |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003737/ |
