PXD068062-1

PXD068062 is an original dataset announced via ProteomeXchange.

Title	A selectivity filter in Tim17 mediates intramitochondrial protein sorting - Data set I
Description	The translocase of the inner membrane of mitochondria (TIM23 complex) imports proteins into the matrix and, in the case of membrane proteins, releases precursors laterally into the inner membrane. The sorting of precursor proteins relies on topogenic signals in their structure. How these signals are deciphered is unknown. Using a fluorescence-based sorting reporter, we systematically screened yeast depletion libraries and identified Dmo2 (akin to DMAC1 in humans) as a novel protein critical for the membrane insertion of inner membrane proteins. Dmo2 serves as oxidoreductase that introduces a disulfide bond into Tim17, the protein-conducting subunit of the TIM23 complex. Molecular dynamics simulations show that the disulfide bond in Tim17 positions the negatively charged N terminus of Tim17 to the protein-conducting vestibule of the TIM23 translocase to constitute a negatively charged selection filter for incoming precursor proteins. Our paper provides the first molecular rationale as to how the TIM23 translocase differentiates between soluble and membrane proteins.
HostingRepository	PRIDE
AnnounceDate	2026-08-03
AnnouncementXML	Submission_2026-08-03_07:52:51.959.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Räschle
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-04 05:52:15	ID requested
⏵ 1	2026-08-03 07:52:52	announced

Dataset with its publication pending

submitter keyword: None

Markus Räschle
contact affiliation	Molecular Genetics, RPTU Kaiserslautern
contact email	raeschle@rhrk.uni-kl.de
lab head
Markus Räschle
contact affiliation	Technical University Kaiserslautern
contact email	raeschle@rhrk.uni-kl.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068062
     1. Label: PRIDE project
     2. Name: A selectivity filter in Tim17 mediates intramitochondrial protein sorting - Data set I