PXD068027 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of RUNX1 binding proteins in control and entinostate treated mouse leukemia cells |
| Description | Runt-related Transcription Factor 1 (RUNX1) is essential for definitive hematopoiesis and is among the most frequently mutated genes in leukemia. Previous work from our lab demonstrated that Histone Deacetylase 1 (HDAC1), a known RUNX1 partner, is unexpectedly required for active transcription suggesting a non-histone role for HDAC1 regulating components of the RUNX1 complex. Here, we use proteomics, genomics, and long-read transcriptomics to identify novel RUNX1 interacting partners and decipher their role in gene regulation and RNA splicing in leukemia cells. We demonstrate that Polypyrimidine Tract Binding Protein 1 (PTBP1) interacts with RUNX1 in an HDAC1 dependent manner. Chromatin profiling revealed extensive genome-wide overlap in sites occupied by RUNX1 and PTBP1, with significant enrichment at promoters of actively transcribed genes. Loss of PTBP1 in AML cells led to widespread alterations in RNA splicing and decreased expression of genes whose promoters are bound by both factors, including metabolic genes. In agreement with these findings, we found that loss of PTBP1 reduced glycolysis and glucose uptake and ultimately caused cell death. Based on our data, we propose that the interaction between RUNX1 and PTBP1 facilitates expression of metabolic proteins essential for leukemia cell growth and survival. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-19 |
| AnnouncementXML | Submission_2026-01-18_16:30:41.350.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | R. Katherine Hyde |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-03 03:53:13 | ID requested | |
| ⏵ 1 | 2026-01-18 16:30:42 | announced | |
Publication List
| Dhir A, Ethell A, Watkins R, Lam C, Tur-Rodriguez K, Persinger J, Dobish KK, Panda S, Buckley SM, Swenson SA, Brahma S, Rowley MJ, Hyde RK, The splicing factor PTBP1 interacts with RUNX1 and is required for leukemia cell survival. Leukemia, 40(1):138-151(2026) [pubmed] |
| 10.1038/s41375-025-02799-w; |
Keyword List
| submitter keyword: HDAC1, leukemia, PTBP1,Runx1, AML |
Contact List
| R. Katherine Hyde |
|---|
| contact affiliation | University of Nebraska Medical Center |
| contact email | kate.hyde@unmc.edu |
| lab head | |
| R. Katherine Hyde |
|---|
| contact affiliation | University of Nebraska Medical Center |
| contact email | kate.hyde@unmc.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD068027 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068027
- Label: PRIDE project
- Name: Identification of RUNX1 binding proteins in control and entinostate treated mouse leukemia cells
