PXD068015-1

PXD068015 is an original dataset announced via ProteomeXchange.

Title	mTORC1 phosphorylates SNX16 in vivo
Description	During autophagy, the contents enclosed within autophagosomes are degraded, while the outer membrane components are recycled from autolysosomes by the recycler complex through the recently discovered autophagosomal components recycling (ACR) process. This recycling is essential for maintaining autophagic activity. However, the molecular machinery and upstream regulatory mechanisms driving this recycling process remain poorly understood. Here, we identify SNX16 as a key component of the recycler complex, which localizes to autolysosomes and is required for ACR. SNX16 functions in ACR by regulating recycler complex formation, facilitating cargo recognition, and mediating the connection between STX17-SNX4-SNX5 and dynein-dynactin complexes. In addition, SNX16-cargo interactions are regulated by two ACR-related small GTPases, Rab32 and Rab38. Importantly, mTORC1 phosphorylates SNX16 to regulate ACR by inhibiting its interactions with STX17 and other recycler components, thus preventing recycler complex formation. Taken together, our findings identify SNX16 as a novel recycler component and establish a link between mTORC1 and ACR.
HostingRepository	iProX
AnnounceDate	2025-09-03
AnnouncementXML	Submission_2025-09-03_00:07:38.148.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huilin Que
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-03 00:06:56	ID requested
⏵ 1	2025-09-03 00:07:38	announced

Dataset with its publication pending

submitter keyword: mTORC1, SNX16 phosphorylation

Yueguang Rong
contact affiliation	Huazhong University of Science and Technology
contact email	rongyueguang@hust.edu.cn
lab head
Huilin Que
contact affiliation	Huazhong University of Science and Technology
contact email	1445965163@qq.com
dataset submitter

iProX dataset URI