PXD068004 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The proteomic landscape of CTNNB1 mutated low-grade early-stage endometrial carcinomas |
| Description | Endometrial carcinoma is the most frequent gynecologic malignancy in western countries. In recent years, mutations in CTNNB1 have been associated with worse prognosis in low-risk carcinomas. However, there is a lack of understanding of the proteomic implications of CTNNB1 mutations in this type of tumor. In this study, we performed shotgun proteomics using Formalin Fixed Paraffin Embedded (FFPE) tissue samples of CTNNB1 mutated and wild type low-risk endometrial carcinomas. A publicly available proteomic and transcriptomic database was used to validate results. Differential protein expression and Gene Set Enrichment Analysis revealed dysregulation of pathways associated with cell keratinization, immune response modulation, and intracellular calcium regulation. CTNNB1 mutated tumors showed immune dysregulation at multiple levels including cytokine secretion, cell adhesion, and lymphocyte activation. These results were supported by tissue multiplex immunofluorescence analysis, demonstrating reduced CD8 tumor infiltrating lymphocytes and different immune spatial interaction patterns. Intracellular calcium dysfunction was associated with key transcript dysregulation. We found an increased expression of CAMK2A and ROR2, suggesting a potential role for non-canonical Wnt pathway activation in CTNNB1 mutated tumors. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-24 |
| AnnouncementXML | Submission_2025-11-23_16:18:56.318.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Alberto Pelaez García |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-09-02 13:24:18 | ID requested | |
| ⏵ 1 | 2025-11-23 16:18:57 | announced | |
Publication List
| 10.3390/cells14211676; |
| Lopez-Janeiro A, Brizzi E, Ruz-Caracuel I, Alexandru R, de Andrea C, Berj, ó, n A, Yebenes L, Mendiola M, Heredia-Soto V, Montero-Calle A, Barderas R, de Los Rios V, Redondo A, Pelaez-Garcia A, Hardisson D, Mutated Low-Grade Early-Stage Endometrial Carcinomas. Cells, 14(21):(2025) [pubmed] |
Keyword List
| submitter keyword: Proteomics |
| Endometrial Carcinoma |
| Wnt pathway |
| Immune microenvironment |
Contact List
| Alberto Pelaez-Garcia |
|---|
| contact affiliation | Proteomics Unit (UCCTs), Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain |
| contact email | alberto.pelaez@isciii.es |
| lab head | |
| Alberto Pelaez García |
|---|
| contact affiliation | Proteomics Core National Institute of health Carlos III |
| contact email | albertopelaez84@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD068004 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD068004
- Label: PRIDE project
- Name: The proteomic landscape of CTNNB1 mutated low-grade early-stage endometrial carcinomas
